通过cDNA克隆实验鉴定的来自石头鱼（毒鲉）毒液中VTXβ亚基的完整氨基酸序列。

Complete amino-acid sequence of the beta-subunit of VTX from venom of the stonefish (Synanceia verrucosa) as identified from cDNA cloning experiments.

作者信息

Garnier P, Ducancel F, Ogawa T, Boulain J C, Goudey-Perrière F, Perrière C, Ménez A

机构信息

Laboratoire de Biologie et Contrôle des Organismes parasites (Equipe Toxines et Insectes), Faculté de Pharmacie, Université de Paris-Sud, Châtenay-Malabry, France.

出版信息

Biochim Biophys Acta. 1997 Jan 4;1337(1):1-5. doi: 10.1016/s0167-4838(96)00187-2.

DOI:10.1016/s0167-4838(96)00187-2

PMID:9003430

Abstract

A cDNA encoding a subunit of the verrucotoxin (VTX) has been identified from a cDNA library derived from stonefish venom glands. It encodes a polypeptide of 708 amino-acid residues, followed by a 3'-untranslated region of 895 bp long. The ORF contains the complete mature sequence of the beta-subunit of the VTX, as inferred from both the presence of an identical N-terminus sequence and 96% homology among the 506 amino terminus residues found in the partial sequence of the beta-subunit of the stonustoxin from Synanceia horrida (Ghadessy, F.J., Jeyaseelan, K., Chung, M.C.M., Khoo, H.E., and Yuen, R. (1994) Toxicon 32, 1684-1688). Upstream the mature sequence, we noticed the presence of an incomplete peptide of a 13 amino acids, whose unusual primary structure supports the idea of the existence of a propeptide and/or of a new secretion signal.

摘要

从石鱼毒腺的cDNA文库中鉴定出一个编码疣毒素（VTX）亚基的cDNA。它编码一个由708个氨基酸残基组成的多肽，后面跟着一个长度为895 bp的3'非翻译区。从在来自 horrida 虎纹钝口螈的石鱼毒素β亚基部分序列中发现的相同N端序列以及506个氨基末端残基间96%的同源性推断，该开放阅读框包含VTXβ亚基的完整成熟序列（Ghadessy, F.J., Jeyaseelan, K., Chung, M.C.M., Khoo, H.E., and Yuen, R. (1994) Toxicon 32, 1684 - 1688）。在成熟序列上游，我们注意到存在一个13个氨基酸的不完整肽段，其不寻常的一级结构支持前肽和/或新分泌信号存在的观点。