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家蚕脂质转运颗粒的纯化及特性:与烟草天蛾脂质转运颗粒的比较。

Purification and properties of a lipid transfer particle from Bombyx mori: comparison to the lipid transfer particle from Manduca sexta.

作者信息

Tsuchida K, Soulages J L, Moribayashi A, Suzuki K, Maekawa H, Wells M A

机构信息

Division of Radiological Protection, National Institute of Health, Tokyo, Japan.

出版信息

Biochim Biophys Acta. 1997 Jan 4;1337(1):57-65. doi: 10.1016/s0167-4838(96)00149-5.

Abstract

A lipid transfer particle (LTP) was purified from the hemolymph of the silkworm Bombyx mori. Like other insect LTPs, the B. mori LTP is a very high density lipoprotein containing 21% lipid and three apoproteins of mass approximately 350 kDa, approximately 85 kDa, and approximately 60 kDa. B. mori LTP catalyzes the exchange of lipids between different density class lipoproteins found in adult hemolymph and between adult lipoproteins and vitellogenin. However, in no case was net lipid transfer observed. Manduca sexta LTP also catalyzed exchange of lipids, but not net transfer of lipids, between different density class lipoproteins found in adult hemolymph.

摘要

从家蚕的血淋巴中纯化出一种脂质转运颗粒(LTP)。与其他昆虫LTP一样,家蚕LTP是一种极高密度脂蛋白,含有21%的脂质和三种质量分别约为350 kDa、约85 kDa和约60 kDa的载脂蛋白。家蚕LTP催化成年血淋巴中不同密度级别的脂蛋白之间以及成年脂蛋白与卵黄原蛋白之间的脂质交换。然而,在任何情况下都未观察到净脂质转运。烟草天蛾LTP也催化成年血淋巴中不同密度级别的脂蛋白之间的脂质交换,但不是脂质的净转运。

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