Hillebrandt S, Streffer C, Müller W U
Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, Germany.
Mutat Res. 1996 Nov 11;372(1):43-51. doi: 10.1016/S0027-5107(96)00109-1.
An inbred mouse strain HLG shows a high incidence of gastroschisis after X-ray exposure to the zygotes. About 11% of the fetuses display this malformation after irradiation with 1 Gy. The C57BL-strain does not show the increased frequency of gastroschisis after radiation-exposure to the zygotes. The genetic background of this malformation was investigated in a backcross of HLG x C57BL females to HLG males. The pregnant HLG x C57BL females were irradiated in a stage in which the (HLG x C57BL) x HLG [BC1] embryos were in the 1-cell stage. The frequency of gastroschisis in the BC1 generation was compared with a genetic model of a single recessive mutation with 11% penetrance. This frequency does not fit a single-locus inheritance. The number of loci involved was estimated to be about two or three. HLG mouse strain may be a valuable animal model in the study of polygenic traits.
一种近交系小鼠品系HLG在对合子进行X射线照射后，腹裂发生率很高。用1 Gy照射后，约11%的胎儿出现这种畸形。C57BL品系在对合子进行辐射暴露后，腹裂频率并未增加。在HLG×C57BL雌性与HLG雄性的回交中研究了这种畸形的遗传背景。怀孕的HLG×C57BL雌性在（HLG×C57BL）×HLG [BC1]胚胎处于单细胞期的阶段接受照射。将BC1代中腹裂的频率与具有11%外显率的单隐性突变遗传模型进行比较。该频率不符合单基因座遗传。估计涉及的基因座数量约为两到三个。HLG小鼠品系可能是研究多基因性状的有价值的动物模型。
