Gossett R, Kier A B, Schroeder F, McConkey D, Fadok V, Amoss M S
Department of Veterinary Pathobiology, Texas A&M University, College Station 77843-4467, USA.
J Comp Pathol. 1996 Nov;115(4):353-72. doi: 10.1016/s0021-9975(96)80071-5.
The role of apoptosis in the spontaneous regression of Sinclair swine melanoma was investigated in vitro with swine melanoma cell lines. Growth characteristics and sensitivity to cycloheximide-induced apoptosis were determined in melanoma cell lines derived from tumours that were progressing or undergoing regression in vivo. In contrast to cell lines derived from progressing tumours, those derived from regressing tumours showed induction of apoptosis; this phenomenon was dependent on dose but independent of cell growth stage in culture. Chromatin condensation, cell shrinkage, and fragmentation into apoptotic bodies, as well as DNA fragmentation into large kilobase fragments, occurred in cell lines from regressing tumours but not from progressing tumours. These findings suggest that swine melanoma cells are inherently more sensitive to cell death during tumour regression. The apoptosis-sensitive and resistant cell lines will be important for further studies of the roles of cell signalling pathways and gene expression in tumour regression.
利用猪黑色素瘤细胞系在体外研究了细胞凋亡在辛克莱猪黑色素瘤自发消退中的作用。测定了源自体内正在进展或正在消退肿瘤的黑色素瘤细胞系的生长特性以及对环己酰亚胺诱导凋亡的敏感性。与源自进展期肿瘤的细胞系不同，源自消退期肿瘤的细胞系表现出凋亡诱导；这种现象取决于剂量，但与培养中的细胞生长阶段无关。在源自消退期肿瘤而非进展期肿瘤的细胞系中，出现了染色质浓缩、细胞收缩、碎裂成凋亡小体以及DNA断裂成大的千碱基片段的现象。这些发现表明，猪黑色素瘤细胞在肿瘤消退过程中对细胞死亡具有内在的更高敏感性。凋亡敏感和抗性细胞系对于进一步研究细胞信号通路和基因表达在肿瘤消退中的作用将具有重要意义。
