Suzuki T, Ohno N, Chiba N, Miura N N, Adachi Y, Yadomae T
Laboratory of Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
J Pharm Pharmacol. 1996 Dec;48(12):1243-8. doi: 10.1111/j.2042-7158.1996.tb03930.x.
Although it has been established that soluble glucan in fungi is important to host defence against infection, the importance of insoluble glucans is not clear. We have examined the in-vivo immunopharmacological activity of the insoluble glucan, zymocel. Administration of zymocel increased peritoneal exudate cell number and spleen weight, and enhanced: phagocytic activity, hydrogen peroxide production, and nitric oxide production of peritoneal exudate cells; the extravascular release of Evans blue (which might reflect vascular permeability); lipopolysaccharide-triggered synthesis of tumour necrosis factor (TNF); and recovery of white blood cell number in cyclophosphamide-induced leukopenia. Zymocel also showed anti-tumour activity against sarcoma 180 in mice and also enhanced TNF synthesis and hydrogen peroxide production by macrophage-like cell line in-vitro, i.e. resulted in direct macrophage activation. These results show that zymocel shows varied immunopharmacological activity; it is suggested that the administration of insoluble glucan induces the inflammatory response, the subsequent activation of the immune systems via the cytokine network, and direct macrophage activation.
虽然已经确定真菌中的可溶性葡聚糖对宿主抵抗感染的防御作用很重要，但不溶性葡聚糖的重要性尚不清楚。我们已经研究了不溶性葡聚糖zymocel的体内免疫药理活性。给予zymocel可增加腹腔渗出细胞数量和脾脏重量，并增强：腹腔渗出细胞的吞噬活性、过氧化氢生成和一氧化氮生成；伊文思蓝的血管外释放（这可能反映血管通透性）；脂多糖触发的肿瘤坏死因子（TNF）合成；以及环磷酰胺诱导的白细胞减少症中白细胞数量的恢复。zymocel对小鼠肉瘤180也显示出抗肿瘤活性，并且在体外还增强了巨噬细胞样细胞系的TNF合成和过氧化氢生成，即导致直接的巨噬细胞激活。这些结果表明zymocel具有多种免疫药理活性；提示给予不溶性葡聚糖可诱导炎症反应，随后通过细胞因子网络激活免疫系统，并直接激活巨噬细胞。
