Aroniadou-Anderjaska V, Lemon P W, Gilloteaux J
Department of Anatomy, University of Maryland, Baltimore 21201-1509, USA.
Tissue Cell. 1996 Dec;28(6):719-24. doi: 10.1016/s0040-8166(96)80074-7.
We examined the effects of exogenous growth hormone (GH) treatment on the soleus and rectus femoris muscles of young female rats. Rat GH (1.8 IU/mg) was administered for 3 weeks by subcutaneous injection, twice a day, at doses of 0.5, 0.6, and 0.8 mg/day during the 1st, 2nd and 3rd week, respectively. Final body weight, as well as wet and dry weight, of the soleus and rectus femoris muscles were significantly greater in the GH-treated group, compared to controls. Muscle weight to body weight ratios did not differ between the two groups. The fiber type composition of the soleus muscle was determined by histochemical staining for myosin ATPase activity. No statistically significant difference was found between the GH-treated and the control groups in the percentages of fiber types. However, GH treatment significantly increased the cross-sectional area of type II fibers of the soleus muscle. These results suggest that, in young female rats, acceleration of body weight gain by homologous GH administration is accompanied by a proportional hypertrophy of skeletal muscle mass. Increased muscle mass is due to hypertrophy of muscle fibers. Type II muscle fibers appear to be more sensitive to GH stimulation.
我们研究了外源性生长激素（GH）治疗对年轻雌性大鼠比目鱼肌和股直肌的影响。通过皮下注射给予大鼠生长激素（1.8 IU/mg），为期3周，每天两次，在第1、2和3周分别给予0.5、0.6和0.8 mg/天的剂量。与对照组相比，GH治疗组的最终体重以及比目鱼肌和股直肌的湿重和干重均显著增加。两组之间的肌肉重量与体重比没有差异。通过肌球蛋白ATP酶活性的组织化学染色确定比目鱼肌的纤维类型组成。GH治疗组和对照组在纤维类型百分比方面未发现统计学上的显著差异。然而，GH治疗显著增加了比目鱼肌II型纤维的横截面积。这些结果表明，在年轻雌性大鼠中，同源GH给药导致体重增加加速的同时，骨骼肌质量也成比例肥大。肌肉质量增加是由于肌纤维肥大。II型肌纤维似乎对GH刺激更敏感。
