高效液相色谱-二极管阵列检测及在线柱后光化学衍生化法在镇痛药测定中的应用
Application of high-performance liquid chromatography with diode-array detection and on-line post-column photochemical derivatization to the determination of analgesics.
作者信息
Di Pietra A M, Gatti R, Andrisano V, Cavrini V
机构信息
Department of Pharmaceutical Sciences, University of Bologna, Italy.
出版信息
J Chromatogr A. 1996 Apr 5;729(1-2):355-61. doi: 10.1016/0021-9673(95)01037-8.
HPLC analyses of pharmaceutical dosage forms containing analgesics and related compounds (acetylsalicyclic acid, paracetamol, propyphenazone, caffeine and chlorpheniramine) were performed on C18 and cyano columns under reversed-phase conditions. The performance of the methods was enhanced by introducing postcolumn on-line photochemical derivatization in combination with a diode-array detection. The column effluents were subjected on-line to UV irradiation (254 nm) and the characteristic photo-induced spectral modifications were useful for the unambiguous identification of the various analgesic compounds. The proposed HPLC methods were successfully applied to the analysis of commercially available analgesic dosage forms.
采用反相条件，在C18柱和氰基柱上对含有镇痛药及相关化合物（乙酰水杨酸、对乙酰氨基酚、保泰松、咖啡因和氯苯那敏）的药物剂型进行了高效液相色谱（HPLC）分析。通过引入柱后在线光化学衍生化结合二极管阵列检测，提高了方法的性能。将柱流出物在线进行紫外线（254 nm）照射，光诱导产生的特征光谱变化有助于明确鉴定各种镇痛化合物。所提出的HPLC方法成功应用于市售镇痛剂型的分析。
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