[The effect of membrane-transport inhibitors on the vacuolization of skeletal muscle fibers induced by glycerin removal from them].

Effects of inhibitors of volume regulation on reversible vacuolation of skeletal muscle transverse sarcotubules have been studied. The drugs include inhibitors of sodium pump (ouabain), electrodiffusion Cl- and K+ channels (SITS, DIDS, niflumic acid, Ba2+), anion-cation cotransport and antiport (bumetanide, furosimide, amiloride), calcium channels (verapamil, nifedipine), stretch-activated ion channels (Gd3+) and water channels (Hg2+). The drugs were used at concentrations usually inhibiting the regulatory volume response in nonmuscle cells. The vacuolation-devacuolation cycles of frog muscle fibers were observed under the light microscope during efflux and entry of glycerol (90 mM). Neither of used inhibitors produced any noticeable effect on development and disappearance of vacuolation. A conclusion is made that development of vacuoles from the T-system during glycerol removal is not associated with activation of ion transport pathways that leads to regulatory volume decrease or increase in other cell types. The mechanisms of T-tubule vacuolation are discussed.