Vavilova G L, Prokopenko O N, Kharlamova O N, Iaremenko M S, Sagach V F
Ukr Biokhim Zh (1978). 1996 Mar-Apr;68(2):42-7.
Activity of transport Na+, K(+)-ATP-ase has been studied both in the ischemia-affected proximal section of the small intestine and in tissues which did not endure ischemia (the brain, renal cortex and the distal part of the small intestine) in dynamics 5, 30 and 60 min, after reperfusion of the ischemized small intestine of rats. It is shown that after 30 min-long ischemia of the small intestine, the Na+, K(+)-ATP-ase activity is inhibited in all the tissues mentioned. Maximal inhibition of the enzyme activity after 30 min long reperfusion is 74, 34, 94 and 58% for the brain, renal cortex, proximal and distal sections of the small intestine, respectively. It has been found that the blood serum from ischemized rats also exerts an inhibitory effect on the enzyme activity. Application of inhibitor of the thrombocyte-activating factor (TAF) BN52021 has permitted demonstrating its role in the protecting effect under conditions of Na+, K(+)-ATP-ase inhibition in the post-Ischemic period and inhibitory effect of the blood serum from ischemized rats on the enzyme activity. A conclusion is made that the TAF participated in the inhibitory effect of Na+, K(+)-ATP-ase activity in different tissues after ischemia of the small intestine of rats.
在大鼠缺血小肠再灌注后的5分钟、30分钟和60分钟动态过程中，研究了Na⁺,K⁺-ATP酶在受缺血影响的小肠近端部分以及未经历缺血的组织（脑、肾皮质和小肠远端）中的活性。结果表明，小肠缺血30分钟后，上述所有组织中的Na⁺,K⁺-ATP酶活性均受到抑制。再灌注30分钟后，该酶活性的最大抑制率在脑、肾皮质、小肠近端和远端分别为74%、34%、94%和58%。研究发现，缺血大鼠的血清对该酶活性也有抑制作用。应用血小板活化因子（TAF）抑制剂BN52021已证实其在缺血后Na⁺,K⁺-ATP酶抑制条件下的保护作用以及缺血大鼠血清对该酶活性的抑制作用。得出的结论是，TAF参与了大鼠小肠缺血后不同组织中Na⁺,K⁺-ATP酶活性的抑制作用。
