Smirnova Iu D, Lushchak V I
Ukr Biokhim Zh (1978). 1996 Mar-Apr;68(2):63-8.
A considerable amount of AMP deaminase (AMPDA) in animal muscles is linked with myofibrils. This link is disturbed with growth of the ionic strength. A smooth increase of AMPDA solubilization has been showed in experiments with rabbit muscles when KCl concentration grew from 0 to 500 mM. Earlier we showed at trout muscles that an increase in KCl concentration from 0 to 200 mM in the homogenization medium induced growth of the bound enzyme portion followed by its decrease at the range of KCl concentrations 300-500 mM. Similar results were obtained in experiments with the sea scorpion muscles, which shows specific character of AMPDA interaction with myofibrils in teleost fish muscles. Comparison of the pH influence on AMPDA extraction from Scorpaena and rabbit muscles supported the idea on the specific character of this enzyme binding in the teleost muscles.
动物肌肉中相当数量的AMP脱氨酶（AMPDA）与肌原纤维相连。这种联系会随着离子强度的增加而受到干扰。在兔肌肉实验中，当氯化钾浓度从0增加到500 mM时，AMPDA的溶解呈平稳增加。早些时候我们在鳟鱼肌肉实验中发现，匀浆介质中氯化钾浓度从0增加到200 mM时，结合酶部分增加，随后在氯化钾浓度300 - 500 mM范围内下降。在海蝎肌肉实验中也得到了类似结果，这表明硬骨鱼肌肉中AMPDA与肌原纤维相互作用具有特异性。比较pH对鲉鱼和兔肌肉中AMPDA提取的影响，支持了硬骨鱼肌肉中这种酶结合具有特异性的观点。
