Møller J F, Robertsen K, Bünger C, Hansen E S
Institute of Experimental Clinical Research, University of Aarhus, Denmark.
Clin Orthop Relat Res. 1997 Jan(334):15-23.
Scanning electron microscopy of methylmethacrylate vascular corrosion casts was used for the morphologic examination of the microvascular system in bone. The methylmethacrylate used for vascular casting was prepared by prepolymerization with ultraviolet light. To approximate the viscosity of blood in the microcirculation, where the hematocrit of blood is lower than in the rest of the circulation, a viscosity between that of plasma and full blood was chosen for the casting material. Neither the ultraviolet prepolymerization of methylmethacrylate nor the choice of a viscosity this low has been used in microcorrosion casting in bone before. Further preparation of the tissue followed 2 directions: (1) complete maceration and decalcification of the specimens, thereby exposing all the vascular structures for detailed examination. By this procedure, it was possible to show detailed and sharp impressions of the vascular bed, and to show, for the first time, the vascular loop from a cutting cone; and (2) maceration without decalcification of the specimens, which is suitable for examination of relations between the microvascular system and the bone tissue. This technique enables studies of the role of the vascular system in bone remodeling and of the involvement of vascular structures in bone disorders. This improved technique for examination of microvascular structures in bone enables detailed studies of the vascular system and its relation to active remodeling sites in bone.
采用甲基丙烯酸甲酯血管铸型扫描电子显微镜对骨组织中的微血管系统进行形态学检查。用于血管铸型的甲基丙烯酸甲酯通过紫外线预聚合制备。为了模拟微循环中血液的粘度（微循环中血液的血细胞比容低于循环系统其他部位），铸型材料选择了介于血浆和全血粘度之间的粘度。甲基丙烯酸甲酯的紫外线预聚合以及如此低粘度的选择此前从未在骨组织的微血管铸型中使用过。组织的进一步处理遵循两个方向：（1）对标本进行完全浸软和脱钙，从而暴露所有血管结构以便进行详细检查。通过该程序，可以清晰显示血管床的详细且清晰的印记，并首次展示来自切割锥的血管袢；（2）对标本进行浸软但不脱钙，这适用于检查微血管系统与骨组织之间的关系。该技术有助于研究血管系统在骨重塑中的作用以及血管结构在骨疾病中的参与情况。这种改进的骨组织微血管结构检查技术能够对血管系统及其与骨组织中活跃重塑部位的关系进行详细研究。
