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在一个Qβ宿主因子(hfq)基因失活的大肠杆菌K12突变体中,噬菌体Qβ的复制大幅减少,但噬菌体MS2的复制正常。

Strongly reduced phage Qbeta replication, but normal phage MS2 replication in an Escherichia coli K12 mutant with inactivated Qbeta host factor (hfq) gene.

作者信息

Su Q, Schuppli D, Winkler M E, Weber H

机构信息

Institut für Molekularbiologie Abt. I, Universität Zürich, Zürich, 8093, Switzerland.

出版信息

Virology. 1997 Jan 6;227(1):211-4. doi: 10.1006/viro.1996.8302.

DOI:10.1006/viro.1996.8302
PMID:9007075
Abstract

RNA phage production was observed in Escherichia coli hfq mutant strains transformed with plasmids containing genomic cDNA of phages Qbeta or MS2. Qbeta production was reduced 5000-fold in an insertional mutant containing an omega cassette in the middle of hfq, but was unaffected in a mutant with the cassette near the end of hfq. The insertions had no effect on MS2 replication.

摘要

在用含有噬菌体Qβ或MS2基因组cDNA的质粒转化的大肠杆菌hfq突变菌株中观察到RNA噬菌体的产生。在hfq中间含有一个ω盒的插入突变体中,Qβ的产生减少了5000倍,但在盒靠近hfq末端的突变体中不受影响。这些插入对MS2复制没有影响。

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