Neuhauss S C, Solnica-Krezel L, Schier A F, Zwartkruis F, Stemple D L, Malicki J, Abdelilah S, Stainier D Y, Driever W
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129, USA.
Development. 1996 Dec;123:357-67. doi: 10.1242/dev.123.1.357.
In a large-scale screen for mutations affecting embryogenesis in zebrafish, we identified 48 mutations in 34 genetic loci specifically affecting craniofacial development. Mutants were analyzed for abnormalities in the cartilaginous head skeleton. Further, the expression of marker genes was studied to investigate potential abnormalities in mutant rhombencephalon, neural crest, and pharyngeal endoderm. The results suggest that the identified mutations affect three distinct aspects of craniofacial development. In one group, mutations affect the overall pattern of the craniofacial skeleton, suggesting that the genes are involved in the specification of these elements. Another large group of mutations affects differentiation and morphogenesis of cartilage, and may provide insight into the genetic control of chondrogenesis. The last group of mutations leads to the abnormal arrangement of skeletal elements and may uncover important tissue-tissue interactions underlying jaw development.
在一项针对影响斑马鱼胚胎发育的突变的大规模筛选中，我们在34个基因位点鉴定出48个特异性影响颅面发育的突变。对突变体的软骨头部骨骼异常进行了分析。此外，研究了标记基因的表达，以调查突变体菱脑、神经嵴和咽内胚层的潜在异常。结果表明，所鉴定的突变影响颅面发育的三个不同方面。在一组中，突变影响颅面骨骼的整体模式，表明这些基因参与了这些元件的特化。另一大组突变影响软骨的分化和形态发生，并可能为软骨形成的遗传控制提供见解。最后一组突变导致骨骼元件的异常排列，并可能揭示颌骨发育背后重要的组织-组织相互作用。
