Ragazzi E, Meggiato C, Chinellato A, Italiano G, Pagano F, Calabrò A
Department of Pharmacology, University of Padova, Italy.
Urol Res. 1996;24(6):323-8. doi: 10.1007/BF00389787.
Transplanted patients frequently present erectile impotence. In order to test any interference by cyclosporine A (CsA), which is commonly used in the post-transplantation management, we investigated the in vitro contractile and relaxant responses of corpus cavernosum and aorta from rabbits chronically treated with CsA. Male New Zealand White rabbits 6 months of age were treated with CsA (25 mg/kg per day s.c.) or solvent (corn oil) for 3 weeks. Descending thoracic aorta and erectile tissue were studied in vitro at the end of treatment. Isometric tension was recorded. In thoracic aorta, noradrenaline (0.1-30 mM) induced a concentration-dependent contraction with no difference between the two groups. Acetylcholine (30 nM-3 mM) produced relaxation (52 +/- 4% at 1 mM) that was significantly reduced in comparison to controls (67 +/- 4%, P < 0.05). ATP (3-10 mM) relaxation was not significantly different (maximal 78 +/- 10% and 62 +/- 12% in CsA-treated and controls). The relaxation produced by sodium nitrite was reduced in CsA-treated rabbits (at 10 mM and 0.1 mM concentrations). In erectile tissue, no significant variation in the response of isolated erectile tissue to the above drugs was observed between CsA-treated and control animals. These data indicate that chronic treatment with CsA in rabbits, despite alteration of the in vitro response of thoracic aorta, does not directly influence the function of penile tissue with relaxants.
移植患者经常出现勃起功能障碍。为了测试常用的移植后管理药物环孢素A（CsA）的任何干扰作用，我们研究了长期用CsA治疗的兔子阴茎海绵体和主动脉的体外收缩和舒张反应。6个月大的雄性新西兰白兔用CsA（每天25mg/kg皮下注射）或溶剂（玉米油）治疗3周。治疗结束时在体外研究降胸主动脉和勃起组织。记录等长张力。在胸主动脉中，去甲肾上腺素（0.1 - 30μM）诱导浓度依赖性收缩，两组之间无差异。乙酰胆碱（30nM - 3mM）产生舒张作用（1mM时为52±4%），与对照组相比显著降低（67±4%，P < 0.05）。ATP（3 - 10mM）舒张作用无显著差异（CsA治疗组和对照组最大分别为78±10%和62±12%）。亚硝酸钠产生的舒张作用在CsA治疗的兔子中降低（在10mM和0.1mM浓度时）。在勃起组织中，CsA治疗组和对照组动物之间，分离的勃起组织对上述药物的反应未观察到显著差异。这些数据表明，兔子长期用CsA治疗，尽管胸主动脉的体外反应发生改变，但对阴茎组织舒张功能没有直接影响。
