Fraisse D, Carnat A, Lamaison J L
Laboratoire de Pharmacognosie et Phytothérapie, Faculté de Pharmacie, Clermont-Ferrand.
Ann Pharm Fr. 1996;54(6):280-3.
Dried leaves of 14 harvested batches and one batch from commercial origine of Vaccinium myrtillus L present a similar polyphenolic pattern. The mean levels of the harvested batches and the levels of the commercial batch were respectively: total polyphenol compounds 12.98 and 10.62%, tannins 7.84 and 7.43%, total flavonoid compounds 2.98 and 2.20% (spectrophotometry), 1.41 and 1.16% (HPLC), quercetin 3-glucuronide 1.02 and 0.83%, hyperoside 0.22 and 0.16%, chlorogenic acid 3.66 and 1.58%. The levels were higher in young leaves and lower in old leaves. A specific chromatographic profile of the flavonoid compounds and a determination method of the tannin or the total polyphenol content were proposed in a standardization purpose.
14个采收批次的欧洲越橘干叶以及一批商业来源的欧洲越橘干叶呈现出相似的多酚谱。采收批次的平均含量以及商业批次的含量分别为:总多酚化合物12.98%和10.62%,单宁7.84%和7.43%,总黄酮化合物2.98%和2.20%(分光光度法)、1.41%和1.16%(高效液相色谱法),槲皮素3 - 葡糖醛酸苷1.02%和0.83%,金丝桃苷0.22%和0.16%,绿原酸3.66%和1.58%。嫩叶中的含量较高,老叶中的含量较低。为了标准化目的,提出了黄酮类化合物的特定色谱图以及单宁或总多酚含量的测定方法。