Castillo D, Neira L, Reid M, Vega P, Fernández N, Urbina A M, González M C, Rodríguez L, Elgueta S
Sub-Departamento de Inmunología, Instituto de Salud Pública de Chile, Santiago de Chile.
Rev Med Chil. 1996 Mar;124(3):342-7.
We measured total serum IgE in 14 patients with allergic diseases and 16 healthy subjects, using three commercial ELISA kits. The correlation of results among the three kits was analyzed using Passing and Bablock regression parameters. Results show that measurements of the different kits do not coincide. One kit shows differences using sera from normal subjects. There is no correlation among kits when using sera from allergic patients. It is concluded that it is not possible to determine exactly the amount of IgE using these kits, specially in subjects with elevated levels.
我们使用三种商用酶联免疫吸附测定（ELISA）试剂盒，对14例过敏性疾病患者和16名健康受试者的血清总免疫球蛋白E（IgE）进行了测量。采用帕辛和巴布洛克回归参数分析了三种试剂盒结果之间的相关性。结果显示，不同试剂盒的测量结果不一致。一种试剂盒在使用正常受试者血清时显示出差异。使用过敏性患者血清时，各试剂盒之间没有相关性。得出的结论是，使用这些试剂盒无法准确测定IgE的量，特别是在IgE水平升高的受试者中。
