Bottazzi C, Demori I, Leone M, Fugassa E
Istituto di Fisiologia Generale, Unviersità di Genova.
Boll Soc Ital Biol Sper. 1996 May-Jun;72(5-6):133-8.
The rat uterus has been shown to be a site of production of insulin-like growth factor-I (IGF-I) and multiple IGF-binding proteins (IGFBP-2, -3, -4, -5, -6) which are involved in estrogen-induced uterine proliferation. The presence of T3-receptors in rat uterus suggests a role of thyroid hormone in the regulation of uterus responses to estradiol. In this study IGF-I and IGFBP-4 mRNAs in uterus, oviduct and cervix from euthyroid, hypothyroid and T3-treated rats were quantified by Northern blot analysis. Our results demonstrate: i) a marked decrease in IGF-I and IGFBP-4 mRNA levels in the uterus but an increase in the oviduct of hypothyroid rats; ii) a marked increase in IGF-I and IGFBP-4 mRNA levels in the uterus but a net decrease in the cervix of T3-treated rats. The uterine changes in IGF-I and IGFBP-4 mRNA levels associated with hypothyroid status were in agreement with those observed in liver.
大鼠子宫已被证明是胰岛素样生长因子-I（IGF-I）和多种IGF结合蛋白（IGFBP-2、-3、-4、-5、-6）的产生部位，这些蛋白参与雌激素诱导的子宫增殖。大鼠子宫中存在T3受体，提示甲状腺激素在调节子宫对雌二醇的反应中起作用。在本研究中，通过Northern印迹分析对正常甲状腺、甲状腺功能减退和T3处理大鼠的子宫、输卵管和子宫颈中的IGF-I和IGFBP-4 mRNA进行了定量。我们的结果表明：i）甲状腺功能减退大鼠子宫中IGF-I和IGFBP-4 mRNA水平显著降低，但输卵管中升高；ii）T3处理大鼠子宫中IGF-I和IGFBP-4 mRNA水平显著升高，但子宫颈中净降低。与甲状腺功能减退状态相关的子宫IGF-I和IGFBP-4 mRNA水平变化与在肝脏中观察到的一致。
