Liu L, Damen J E, Ware M, Hughes M, Krystal G
Terry Fox Laboratory, British Columbia Cancer Research Centre, Vancouver, Canada.
Leukemia. 1997 Feb;11(2):181-4. doi: 10.1038/sj.leu.2400559.
We recently purified and cloned the cDNAs for the murine and human forms of a novel 145 kDa inositol polyphosphate 5-phosphatase (5-ptase) that becomes tyrosine phosphorylated and associated with Shc following stimulation of hemopoietic cells with multiple cytokines. Unlike most 5-ptases which hydrolyze phosphatidylinositol-4,5-P2-bisphosphate (PI-4,5-P2) and/or inositol-1,4,5-trisphosphate (I-1,4,5-P3), this enzyme selectively hydrolyzes the 5'-phosphate from inositol-1,3,4,5-tetraphosphate (I-1,3,4,5-P4) and phosphatidylinositol-3,4,5-trisphosphate (PI-3,4,5-P3), two inositol polyphosphates recently implicated in growth factor-mediated signalling. This 5-ptase is also unique among 5-ptases in that it is the only one to date to possess an SH2 domain. In this review we discuss the cloning, the Shc binding and the potential role of this protein, which we call SHIP, for SH2-containing inositol 5-phosphatase, in cell proliferation, differentiation and apoptosis.
我们最近纯化并克隆了小鼠和人类新型145 kDa肌醇多磷酸5-磷酸酶（5-ptase）的cDNA，该酶在多种细胞因子刺激造血细胞后会发生酪氨酸磷酸化并与Shc结合。与大多数水解磷脂酰肌醇-4,5-二磷酸（PI-4,5-P2）和/或肌醇-1,4,5-三磷酸（I-1,4,5-P3）的5-ptase不同，这种酶选择性地从肌醇-1,3,4,5-四磷酸（I-1,3,4,5-P4）和磷脂酰肌醇-3,4,5-三磷酸（PI-3,4,5-P3）中水解5'-磷酸，这两种肌醇多磷酸最近被认为与生长因子介导的信号传导有关。这种5-ptase在5-ptase中也是独特的，因为它是迄今为止唯一拥有SH2结构域的。在这篇综述中，我们讨论了这种我们称为SHIP（含SH2结构域的肌醇5-磷酸酶）的蛋白质的克隆、与Shc的结合及其在细胞增殖、分化和凋亡中的潜在作用。
