Ma D D, Wei A Q
Department of Haematology, Royal North Shore Hospital, St Leonards, NSW, Australia.
Leuk Res. 1996 Nov-Dec;20(11-12):925-30. doi: 10.1016/s0145-2126(96)00062-8.
The ability of pH-sensitive liposomes and immunoliposomes to deliver synthetic antisense oligonucleotides (oligos) into human myeloid and lymphoid leukaemia cells was examined. The cellular uptake of an 18mer anti-myb oligonucleotide encapsulated in liposomes was from three- to five-fold higher than that of 32P-oligos alone. In addition, anti-CD32 or anti-CD2 immunoliposomes improved the delivery of oligos to leukaemic cells carrying the appropriate receptor for the specific antibody-linked immunoliposome. The uptake of oligos was twice that of the liposome or non-specific immunoliposome encapsulated oligos. These findings support the use of liposomes or immunoliposomes to deliver antisense oligos into human leukaemic cells.
研究了pH敏感脂质体和免疫脂质体将合成反义寡核苷酸（oligos）递送至人髓系和淋巴系白血病细胞的能力。包裹在脂质体中的18聚体抗myb寡核苷酸的细胞摄取量比单独的32P - oligos高3至5倍。此外，抗CD32或抗CD2免疫脂质体改善了oligos向携带特定抗体连接免疫脂质体相应受体的白血病细胞的递送。oligos的摄取量是脂质体或非特异性免疫脂质体包裹的oligos的两倍。这些发现支持使用脂质体或免疫脂质体将反义oligos递送至人白血病细胞。
