Audrain M A, Baranger T A, Moguilevski N, Martin S J, Devys A, Lockwood C M, Muller J Y, Esnault V L
Laboratoire d'Immunologie, CHU Nantes, France.
Clin Exp Immunol. 1997 Jan;107(1):127-34. doi: 10.1046/j.1365-2249.1997.d01-895.x.
Myeloperoxidase (MPO) is one of the main antigen targets of anti-neutrophil cytoplasmic antibodies (ANCA) in systemic vasculitides. It has been suggested that anti-MPO antibodies may recognize a single epitope on recombinant MPO. If confirmed on native MPO, this might allow specific therapeutic intervention with anti-idiotypic MoAbs to prevent antibody antigen interaction which is thought to cause activation of neutrophils and vasculitis. We searched for restriction in the epitope recognition profile in 50 patients with anti-MPO autoantibodies, using both native and recombinant MPO. Mouse monoclonals were purified and tested in competition assays. At least four epitopes were identified on native MPO using these monoclonals and only two were conserved on recombinant MPO. We found that human MPO autoantibody response was not restricted to a single epitope on native MPO, as all sera tested did not show the same profile in competitive studies with monoclonals. Furthermore, 30% of human anti-native MPO sera failed to recognize rMPO.
髓过氧化物酶（MPO）是系统性血管炎中抗中性粒细胞胞浆抗体（ANCA）的主要抗原靶点之一。有人提出，抗MPO抗体可能识别重组MPO上的单个表位。如果在天然MPO上得到证实，这可能允许使用抗独特型单克隆抗体进行特异性治疗干预，以防止抗体与抗原相互作用，这种相互作用被认为会导致中性粒细胞活化和血管炎。我们使用天然MPO和重组MPO，对50例抗MPO自身抗体患者的表位识别谱进行了研究。纯化小鼠单克隆抗体并在竞争试验中进行检测。使用这些单克隆抗体在天然MPO上鉴定出至少四个表位，而在重组MPO上仅两个表位保守。我们发现，人类MPO自身抗体反应并不局限于天然MPO上的单个表位，因为在与单克隆抗体的竞争研究中，所有检测的血清并未显示出相同的谱型。此外，30%的人类抗天然MPO血清无法识别重组MPO。
