Choate J V, Resko J A
Department of Physiology and Pharmacology, Oregon Health Sciences University, Portland 97201, USA.
J Steroid Biochem Mol Biol. 1996 Nov;59(3-4):315-22. doi: 10.1016/s0960-0760(96)00122-7.
To study the tissue-specific control of androgen receptors by circulating androgens, guinea pigs were castrated or castrated and treated with crystalline testosterone propionate. Levels of serum androgens and LH were measured and brain and peripheral tissues were collected for determination of androgen receptor levels under differing androgen states. We found that circulating androgen levels changed rapidly following castration or treatment with exogenous androgen. LH secretion was coupled to circulating androgens; high levels of androgen quickly suppressed LH secretion, whereas LH levels did not rise significantly above intact levels until 7 days following castration. Androgen receptor levels were effected by circulating androgens. Cytosolic androgen receptors (ARc) increased significantly in the preoptic area (POA), septum, anterior pituitary, prostate and seminal vesicle following castration, whereas nuclear androgen receptors (ARn) decreased significantly in the POA, septum, medial basal hypothalamus (MBH), amygdala, parietal cortex, pituitary, prostate and seminal vesicle. Exogenous androgens, which increased serum steroid levels significantly above that in intact animals, decreased ARc below control levels in MBH, amygdala, pituitary and seminal vesicle. High circulating androgens increased ARn above intact levels in the MBH, pituitary and prostate. It thus appears that circulating androgens regulate LH secretion and have profound, but tissue-specific effects on androgen receptors in the guinea pig.
为了研究循环雄激素对雄激素受体的组织特异性调控，对豚鼠进行去势或去势后用结晶丙酸睾酮进行处理。测定血清雄激素和促黄体生成素（LH）水平，并收集脑和外周组织以测定不同雄激素状态下的雄激素受体水平。我们发现，去势或用外源性雄激素处理后，循环雄激素水平迅速变化。LH分泌与循环雄激素相关；高水平的雄激素迅速抑制LH分泌，而直到去势后7天，LH水平才显著高于完整状态下的水平。雄激素受体水平受循环雄激素影响。去势后，视前区（POA）、隔区、垂体前叶、前列腺和精囊中的胞质雄激素受体（ARc）显著增加，而POA、隔区、内侧基底下丘脑（MBH）、杏仁核、顶叶皮质、垂体、前列腺和精囊中核雄激素受体（ARn）显著减少。外源性雄激素使血清类固醇水平显著高于完整动物，使MBH、杏仁核、垂体和精囊中的ARc低于对照水平。高循环雄激素使MBH、垂体和前列腺中的ARn高于完整水平。因此，循环雄激素似乎调节LH分泌，并对豚鼠的雄激素受体有深刻但组织特异性的影响。
