Amiot F, Bellkaid Y, Lebastard M, Ave P, Dautry F, Milon G
CNRS UPR 9044, Institut de Recherches sur le Cancer, Villejuif, France.
Eur Cytokine Netw. 1996 Dec;7(4):733-9.
In this study we further characterized the phenotype at the homeostasis of mice genetically deficient in Tumor Necrosis Factor-alpha and Lymphotoxin-alpha (LT-alpha TNF-alpha -/-). As initially observed in LT-alpha -/- mice, these mice are devoid of lymph nodes and Peyer's patches, while in their spleen the white and red pulp domains are no more detectable. In the blood the leukocytosis dominated by lymphocytosis is not solely due to the absence of lymph nodes. Indeed, this abnormality was shown to be correctable by the transfer of wild type bone marrow in the absence of lymph node. We now report that the metallophilic macrophages of the marginal zone are no more detectable with an antibody reactive to sialoadhesin, a macrophage restricted transmembrane molecule known to bind myeloid and lymphoid cells. The absence of sialoadhesin within the marginal zone, a critical domain for lymphocyte trafficking towards the white pulp suggests a possible cellular basis for the observed blood leukocytosis. In addition, in the peritoneal cavity of LT-alpha TNF-alpha-/- mice, the size of the resident leukocyte population is increased. By their amplitudes these leukocytosis are similar within the blood and the peritoneal compartments.
在本研究中，我们进一步对基因敲除肿瘤坏死因子-α和淋巴毒素-α（LT-α TNF-α -/-）的小鼠在稳态时的表型进行了特征描述。正如最初在LT-α -/-小鼠中所观察到的那样，这些小鼠没有淋巴结和派尔集合淋巴结，而在其脾脏中，白髓和红髓区域也不再能被检测到。在血液中，以淋巴细胞增多为主的白细胞增多症并非仅仅是由于没有淋巴结所致。事实上，在没有淋巴结的情况下，通过移植野生型骨髓可纠正这种异常。我们现在报告，用对唾液酸黏附素具有反应性的抗体无法再检测到边缘区的亲金属巨噬细胞，唾液酸黏附素是一种已知可结合髓样细胞和淋巴细胞的巨噬细胞限制性跨膜分子。边缘区内唾液酸黏附素的缺失，这是淋巴细胞向白髓迁移的关键区域，提示了所观察到的血液白细胞增多症可能的细胞基础。此外，在LT-α TNF-α -/-小鼠的腹腔中，驻留白细胞群体的大小增加。从其幅度来看，这些白细胞增多症在血液和腹腔隔室中是相似的。
