Walters E, Grillo M, Oestreicher A B, Margolis F L
Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, DC 20059, USA.
Int J Dev Neurosci. 1996 Nov;14(7-8):813-22. doi: 10.1016/s0736-5748(96)00063-9.
The ontogeny and cellular specificity of expression of beta-galactosidase activity and olfactory marker protein (OMP) are compared in olfactory tissue of the H-OMP-lacZ-3 line of transgenic mice. In this line the expression of lacZ is driven by a 0.3 kb fragment of the rat OMP promoter. During fetal development, lacZ expression is detectable in olfactory receptor neurons (ORNs) shortly after the initial appearance of endogenous OMP. The beta-galactosidase marker was observed only in mature olfactory receptor neurons where it co-localized with endogenous OMP. It was absent from immature neurons that express the growth associated phosphoprotein B50/GAP43. Lesion of the peripheral olfactory pathway by intranasal irrigation with Triton X-100 eliminated expression of both OMP and lacZ in the olfactory neuroepithelium. Subsequent regeneration of the full complement of olfactory receptor neurons was associated with co-expression of both OMP and beta-galactosidase activity. Neither OMP nor beta-galactosidase activity was induced in any other cell type of the regenerating olfactory mucosa. Thus, as little as 0.3 kb of the OMP promoter has the ability to target lacZ expression to olfactory receptor neurons in a temporally and spatially defined manner. We discuss the potential utility of this transgenic line for future studies of the olfactory system.
在转基因小鼠H-OMP-lacZ-3品系的嗅觉组织中，对β-半乳糖苷酶活性和嗅觉标记蛋白（OMP）表达的个体发生及细胞特异性进行了比较。在该品系中，lacZ的表达由大鼠OMP启动子的0.3 kb片段驱动。在胎儿发育期间，在内源性OMP首次出现后不久，即可在嗅觉受体神经元（ORN）中检测到lacZ表达。β-半乳糖苷酶标记仅在成熟的嗅觉受体神经元中观察到，且与内源性OMP共定位。在表达生长相关磷蛋白B50/GAP43的未成熟神经元中则没有。通过用Triton X-100鼻内冲洗损伤外周嗅觉通路，消除了嗅觉神经上皮中OMP和lacZ的表达。随后嗅觉受体神经元全部补充的再生与OMP和β-半乳糖苷酶活性的共表达相关。在再生的嗅觉黏膜的任何其他细胞类型中均未诱导出OMP或β-半乳糖苷酶活性。因此，仅0.3 kb的OMP启动子就有能力以时间和空间限定的方式将lacZ表达靶向嗅觉受体神经元。我们讨论了该转基因品系在未来嗅觉系统研究中的潜在用途。
