Krivandin A V, Fedorovich I B, Ostrovski'i M A, Fe'igin L A
Biofizika. 1996 Sep-Oct;41(5):1068-74.
The structure of unbleached bovine retinal rod photoreceptor membranes isolated in ficoli density gradient has been studied by means of small-angle X-ray diffraction methods. Samples were prepared in the form of thin multilamellar films of photoreceptor membranes in phase-separated state induced by partial dehydration. Diffraction data were collected using diffractometer with linear position-sensitive detector. Phase signs of structure amplitude were determined by method [7] and membrane lamellar electron density distribution was calculated at 1, 7 nm resolution. The results obtained showed photoreceptor membranes isolated in ficoli density gradient to have asymmetric structure which differed from that of photoreceptor membranes isolated in sucrose density gradient [1]. The asymmetry observed may be accounted for different content of lipid L alpha and L beta phases in cytoplasmic and intradisk membrane layers. It may be assumed that ficoli helps to support membrane native structure.
采用小角X射线衍射方法研究了在菲可利密度梯度中分离得到的未漂白牛视网膜视杆光感受器膜的结构。样品制备成通过部分脱水诱导处于相分离状态的光感受器膜的薄多层膜形式。使用带有线性位置敏感探测器的衍射仪收集衍射数据。通过方法[7]确定结构振幅的相位符号，并在1、7nm分辨率下计算膜层状电子密度分布。所得结果表明，在菲可利密度梯度中分离得到的光感受器膜具有不对称结构，这与在蔗糖密度梯度中分离得到的光感受器膜不同[1]。观察到的不对称性可能是由于细胞质膜层和盘内膜层中脂质Lα和Lβ相的含量不同。可以推测，菲可利有助于维持膜的天然结构。
