Gribanov P G, Shcherbakov A V, Perevozchikova N A, Gusev A A
Biokhimiia. 1996 Jun;61(6):1064-70.
Aerosil A-300 and GF/F (GF/C) filters were used for purifying plasmid DNA and intact RNA as well as for the recovery of DNA fractionated on agarose gels. In the presence of guanidinium thiocyanate Aerosil A-300 and GF/F (GF/C) filters selectively bind nucleic acids, while proteins, agarose polysaccharides and salts are washed off. Nucleic acids desorbed from the Aerosil and filters are available immediately as substrates: DNA for restriction analysis, ligation and sequencing; RNA--for RT-PCR.
亲水性气相二氧化硅A - 300和GF/F（GF/C）过滤器用于纯化质粒DNA和完整RNA，以及回收在琼脂糖凝胶上分离的DNA片段。在硫氰酸胍存在的情况下，亲水性气相二氧化硅A - 300和GF/F（GF/C）过滤器能选择性地结合核酸，而蛋白质、琼脂糖多糖和盐则被洗脱。从亲水性气相二氧化硅和过滤器上解吸的核酸可立即作为底物使用：DNA用于限制性分析、连接和测序；RNA用于逆转录聚合酶链反应（RT-PCR）
