Reichelt B, Niemann H
Institut für Tierzucht und Tierverhalten (FAL) Mariensee, Neustadt.
Dtsch Tierarztl Wochenschr. 1996 Aug-Sep;103(8-9):340-8.
The production of monozygotic twins/multiplets in livestock animal can be achieved either by microsurgical bisection of embryos at the morular- or blastocyst stage, isolation and proliferation of blastomeres from early cleavage stages or nuclear transfer. While the success rates of micro-surgical bisection are high in ruminants (pregnancy rates approximately 50%, twinning rates 20-40%) in polyovulatory species such as swine, the efficiency is low with an average of 20% embryonic survival and 2% monozygotic twins that can positively identified via DNA-fingerprinting. Isolated blastomeres from multicellular embryos still possess great developmental capacity in vitro to progress to the blastocyst stage. However, their development in vivo is markedly reduced. This article summarizes the results obtained by the authors during several years of investigation. The results show for the first time that identical twins can be obtained in pigs which have been demonstrated to be a useful tool in biomedical research.
在家畜中产生单卵双胞胎/多胞胎可通过以下方法实现：在桑葚胚或囊胚阶段对胚胎进行显微手术分割、从早期卵裂阶段分离并增殖卵裂球或进行核移植。虽然在多排卵物种如猪中，显微手术分割的成功率较低，平均胚胎存活率为20%，通过DNA指纹鉴定能确定的单卵双胞胎率为2%，但在反刍动物中显微手术分割的成功率较高（妊娠率约为50%，双胎率为20 - 40%）。从多细胞胚胎中分离出的卵裂球在体外仍具有很强的发育能力，可发育到囊胚阶段。然而，它们在体内的发育明显减弱。本文总结了作者多年研究所得的结果。结果首次表明，在猪中可以获得同卵双胞胎，这已被证明是生物医学研究中的一种有用工具。
