Turmanidze T S, Kvesitadze G I, Mikanadze I U, Shanidze M G, Loladze M Z, Birkadze T V, Alibegashvili M G
Prikl Biokhim Mikrobiol. 1996 Sep-Oct;32(5):519-23.
A scheme for purification of hyaluronidase from Staphylococcus aureus 0-15 that allows to produce a 550-fold-purified preparation with a high specific activity was developed. Hyaluronidase was found to consist of two molecular forms with different molecular weights. Staphylococcus hyaluronidase referred to as a complex preparation and its molecular forms have action optimum at pH 5.0-7.2 and at a temperature between 30 and 37 degrees C. The enzymes were highly specific to their substrate: they catalyzed the depolimerization of hyaluronic acid but did not split the other glycosaminoglycans.
开发了一种从金黄色葡萄球菌0-15中纯化透明质酸酶的方案，该方案能够制备出比活性高、纯化倍数达550倍的制剂。发现透明质酸酶由两种分子量不同的分子形式组成。被称为复合制剂的葡萄球菌透明质酸酶及其分子形式在pH 5.0-7.2和30至37摄氏度的温度下具有最佳活性。这些酶对其底物具有高度特异性：它们催化透明质酸的解聚，但不分解其他糖胺聚糖。
