Semashko M I
Arkh Anat Gistol Embriol. 1977 Jul;73(7):37-41.
A comparative study of four histochemical methods of estimation of noradrenaline- and adrenaline-secreting cells in the adrenal medulla of laboratory animals and man was carried out at different terms after death and depending on the temperature of tissue storage before their histochemical treatment. It was stated that light histochemical method of Honoré and luminescent method of El-Badawi and Schenk are highly specific and make it possible to estimate the amount and location of catecholamines in the adrenal medulla of man and animals. The advantage of honorés method is that it allows to differentiate, at light level, noradrenaline-secreting cells from adrenaline-secreting ones and consider their structural peculiarities. /ence, this method can be recommended for differential study of catecholamines in the adrenal medulla of laboratory animals and man.
对实验动物和人类肾上腺髓质中去甲肾上腺素和肾上腺素分泌细胞的四种组织化学估计方法进行了比较研究,研究在死后不同时间进行,并取决于组织在进行组织化学处理前的储存温度。结果表明,奥诺雷的光组织化学方法以及埃尔-巴达维和申克的发光方法具有高度特异性,能够估计人和动物肾上腺髓质中儿茶酚胺的含量和位置。奥诺雷方法的优点在于,它能够在光学水平上区分去甲肾上腺素分泌细胞和肾上腺素分泌细胞,并观察它们的结构特点。因此,该方法可推荐用于实验动物和人类肾上腺髓质中儿茶酚胺的差异研究。