Kniazev G G, Spiridonov V K
Arkh Anat Gistol Embriol. 1977 Jul;73(7):89-93.
While using Falck's method, a number of simple and handy additional ways were suggested to carry on quantitative estimation of the fluorescence change of noradrenergic and dopaminergic terminals in a number of nuclei of the central nervous system. A device was used, which allows to obtain samples of brain of a given size and in a definite plane. The method we used makes it possible to treat simultaneously a great number of material at all the stages of work. Fluorescence intensity was measured by means of fluorometric device with the stop of a large diameter. Tests with the substances lowering the content of catecholamines showed that at the content of catecholamine less than 60% one can observe linear dependance between fluorescence intensity and the content of catecholamine in tissues. In the sphere of high concentrations such linearity does not exist.
在使用法尔克氏方法时,有人提出了一些简单且便捷的额外方法,用于对中枢神经系统多个核团中去甲肾上腺素能和多巴胺能终末的荧光变化进行定量估计。使用了一种装置,该装置能够获取给定大小且在特定平面的脑样本。我们所采用的方法使得在工作的各个阶段能够同时处理大量材料。通过带有大直径光阑的荧光测量装置来测量荧光强度。用降低儿茶酚胺含量的物质进行的测试表明,当儿茶酚胺含量低于60%时,可以观察到荧光强度与组织中儿茶酚胺含量之间的线性关系。在高浓度范围内不存在这种线性关系。