Ulich T R, Whitcomb L, Tang W, O'Conner Tressel P, Tarpley J, Yi E S, Lacey D
Amgen, Inc., Thousand Oaks, California 91320, USA.
Cancer Res. 1997 Feb 1;57(3):472-5.
To determine whether keratinocyte growth factor (KGF), an epithelial and urothelial growth factor, ameliorates cyclophosphamide (CP)-induced cystitis in rats, KGF (5 mg/kg) was injected in rats as a single i.v. injection 24 h prior to i.p. injection of CP (200 mg/kg). Bladders were evaluated histologically 48 h after CP injection, and KGF pretreatment was found to almost completely prevent CP-induced ulcerative hemorrhagic cystitis. Urinary KGF levels were measured by ELISA, and KGF was found to be undetectable in control urine, but it was found to appear in the urine of KGF-treated rats at 8 h, with a peak concentration of approximately 10 ng/ml. Bilateral nephrectomy did not diminish the proliferative effect of KGF on urothelium, suggesting that the contribution of urinary KGF to urothelial proliferation is insignificant. In conclusion, systemic administration of KGF is protective against CP-induced cystitis. Although KGF appears in the urine, urinary KGF is not necessary for the proliferative action of KGF on urothelium.
为了确定角质形成细胞生长因子（KGF）（一种上皮和尿路上皮生长因子）是否能改善环磷酰胺（CP）诱导的大鼠膀胱炎，在腹腔注射CP（200mg/kg）前24小时，给大鼠静脉内单次注射KGF（5mg/kg）。在注射CP后48小时对膀胱进行组织学评估，发现KGF预处理几乎完全预防了CP诱导的溃疡性出血性膀胱炎。通过酶联免疫吸附测定法测量尿KGF水平，发现在对照尿液中检测不到KGF，但在KGF处理的大鼠尿液中8小时时可检测到，峰值浓度约为10ng/ml。双侧肾切除并没有减弱KGF对尿路上皮的增殖作用，这表明尿KGF对尿路上皮增殖的作用不显著。总之，全身性给予KGF对CP诱导的膀胱炎具有保护作用。虽然KGF出现在尿液中，但尿KGF对于KGF对尿路上皮的增殖作用并非必需。
