棉酚处理后生精细胞中细胞微管蛋白和微管微管蛋白含量的可逆变化。

Reversible changes in the content of cellular and microtubular tubulin in spermatogenic cells after gossypol treatment.

作者信息

Teng C S

机构信息

North Carolina State University, Department of Anatomy, Physiological Sciences and Radiology, Raleigh 27606, USA.

出版信息

Contraception. 1997 Jan;55(1):41-6. doi: 10.1016/s0010-7824(96)00240-5.

DOI:10.1016/s0010-7824(96)00240-5

PMID:9013060

Abstract

After exposure of young male rats to gossypol acetic acid for various times, a reduction in the content of cellular and microtubular beta-tubulin was found in spermatocytes and spermatids. The content of tubulin was measured by enzyme-linked immunosorbent assay (ELISA). The results were expressed as micrograms tubulin/100 micrograms total protein and compared with those of the control rats. After drug treatment for 2, 6, 12, and 20 weeks, the content of total cell tubulin in spermatocyte was reduced by 2.4%. 8.8%. 52%, and 61%, respectively, whereas the content of tubulin in spermatid was reduced by 7.4%, 36%, 70%, and 72%, respectively. At the same time length of drug treatment, the content of microtubular tubulin in spermatocyte was reduced by 1.6% 13%, 58% and 61% in comparison to the reduction rate of 5%, 37%, 69%, and 77%, respectively, for spermatid. These results indicated that the tubulin associated with spermatids were more vulnerable to gossypol than that of the spermatocytes. Eight weeks after withdrawal of the drug treatment, the content of tubulin in spermatocytes and spermatids was recovered.

摘要

将年轻雄性大鼠暴露于醋酸棉酚不同时间后，发现精母细胞和精子细胞中细胞微管β-微管蛋白含量降低。微管蛋白含量通过酶联免疫吸附测定（ELISA）法测量。结果以微克微管蛋白/100微克总蛋白表示，并与对照大鼠的结果进行比较。药物处理2、6、12和20周后，精母细胞中总细胞微管蛋白含量分别降低了2.4%、8.8%、52%和61%，而精子细胞中微管蛋白含量分别降低了7.4%、36%、70%和72%。在相同的药物处理时间下，精母细胞中微管微管蛋白含量降低了1.6%、13%、58%和61%，而精子细胞的降低率分别为5%、37%、69%和77%。这些结果表明，与精子细胞相关的微管蛋白比精母细胞的微管蛋白更容易受到棉酚的影响。药物处理停药8周后，精母细胞和精子细胞中微管蛋白含量恢复。