使用埃特拉（Extrelut）柱对生物材料中的甲基苯丙胺及其代谢物进行简单的萃取衍生化，用于气相色谱-质谱联用（GC-MS）测定。
Simple extractive derivatization of methamphetamine and its metabolites in biological materials with Extrelut columns for their GC-MS determination.
作者信息
Hara K, Kashimura S, Hieda Y, Kageura M
机构信息
Department of Forensic Medicine, Fukuoka University School of Medicine, Japan.
出版信息
J Anal Toxicol. 1997 Jan-Feb;21(1):54-8. doi: 10.1093/jat/21.1.54.
A simple, extractive heptafluoro-n-butyrylation with Extrelut columns was devised to simultaneously measure methamphetamine (MAMP), amphetamine (AMP), 4-hydroxymethamphetamine (HMAMP), and 4-hydroxyamphetamine (HAMP) in biological materials by gas chromatography-mass spectrometry (GC-MS) using 4-methoxymethamphetamine-d5 as the internal standard. Human urine, human whole blood, and porcine skeletal muscle spiked with the stimulant standards were used for evaluating the method. After deproteinization and adjustment of the pH to 12.6, the sample was applied to an Extrelut column. Using the present method, AMP, MAMP, and HMAMP could be determined in an actual forensic case study.
设计了一种使用埃特拉卢特柱的简单萃取七氟正丁酰化方法，以4-甲氧基甲基苯丙胺-d5作为内标，通过气相色谱-质谱联用（GC-MS）同时测定生物材料中的甲基苯丙胺（MAMP）、苯丙胺（AMP）、4-羟基甲基苯丙胺（HMAMP）和4-羟基苯丙胺（HAMP）。用添加了兴奋剂标准品的人尿液、人全血和猪骨骼肌来评估该方法。在进行脱蛋白处理并将pH值调至12.6后，将样品应用于埃特拉卢特柱。使用本方法，在一个实际的法医案例研究中可以测定AMP、MAMP和HMAMP。
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