平衡温度对小鼠受精卵冷冻保存过程中丙二醇毒性的有害影响。
Deleterious effect of equilibration temperature on the toxicity of propanediol during cryopreservation of mouse zygotes.
作者信息
Mahadevan M M, Miller M M
机构信息
Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
出版信息
J Assist Reprod Genet. 1997 Jan;14(1):51-4. doi: 10.1007/BF02765753.
PURPOSE
Our aim was to determine the effect of temperature and incubation time on the toxicity of propanediol on mouse zygotes.
METHOD
Zygotes were pooled and randomly allocated to one of the treatment groups. Zygotes were incubated in PBS or PBS containing 1.5M propanediol at 37 degrees C or room temperature (RT) for up to 30 min, washed, and cultured. Similarly, zygotes were incubated at 4 degrees C or RT for 30 min and either washed and cultured or frozen-thawed, washed, and cultured. Zygotes were examined at 24, 48, and 96 hr of culture and embryo quality was determined.
RESULTS
Exposure of zygotes to 1.5 M propanediol at 37 degrees C significantly impaired first cleavage and blastocyst formation compared to exposure at RT (P < 0.0007). Incubation of zygotes for more than 5 min at 37 degrees C significantly reduced embryo development (P < 0.00001). Exposure to propanediol at 4 degrees C results in similar embryo development before and after freeze-thawing compared to exposure at RT.
CONCLUSIONS
Propanediol is toxic to mouse zygotes in a temperature- and time-dependent fashion. Cryopreservation of zygotes after exposure at 4 degrees C appears to be no better than after exposure at RT.
目的
我们的目的是确定温度和孵育时间对丙二醇对小鼠受精卵毒性的影响。
方法
将受精卵汇集并随机分配到其中一个处理组。将受精卵在37℃或室温（RT）下于PBS或含有1.5M丙二醇的PBS中孵育长达30分钟，洗涤后进行培养。同样，将受精卵在4℃或RT下孵育30分钟，然后要么洗涤后培养，要么冻融、洗涤后培养。在培养24、48和96小时时检查受精卵，并确定胚胎质量。
结果
与在室温下暴露相比，在37℃下将受精卵暴露于1.5M丙二醇显著损害了首次卵裂和囊胚形成（P < 0.0007）。在37℃下将受精卵孵育超过5分钟显著降低了胚胎发育（P < 0.00001）。与在室温下暴露相比，在4℃下暴露于丙二醇导致冻融前后的胚胎发育相似。
结论
丙二醇对小鼠受精卵具有温度和时间依赖性毒性。在4℃下暴露后对受精卵进行冷冻保存似乎并不比在室温下暴露后更好。
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