Dickerson R N, Brown R O, Mouser J F, Kuhl D A, Hak E B, Methvin J T, Hak L J
Department of Clinical Pharmacy, University of Tennessee, Memphis 38163, USA.
J Am Coll Nutr. 1997 Feb;16(1):74-80. doi: 10.1080/07315724.1997.10718652.
This study compared the effect of different doses of octreotide on glucose and protein homeostasis in rats receiving concomitant lipopolysaccharide and parenteral nutrition infusions.
Sixty-six male Sprague Dawley rats (185 to 220 g) were randomized to receive parenteral nutrition only (PN), PN plus continuous infusion of Escherichia coli 026:B6 lipopolysaccharide at 6 mg/kg/day (LPS), PN plus LPS plus octreotide at 10 micrograms/kg/day (LPS + Oct 10), 100 micrograms/kg/day (LPS + Oct 100), or 1000 micrograms/kg/day (LPS + Oct 1000) for 48 hours. Prior to randomization all animals received isocaloric and isonitrogenous PN (170 kcal/kg/day as glucose and 1.1 g N/kg/day) and were kept nil per os except for water ad libitum. Nitrogen balance, urinary 3-methylhistidine/creatinine ratio, serum glucose concentration, and incidence of glycosuria were compared between groups. Serum urea nitrogen (SUN) changes were incorporated into the cumulative 48 hour nitrogen balance. ANOVA, Duncan's multiple range test, and Fisher's Exact Test were used for statistical analysis.
Nitrogen balance (mg/48 hours) was significantly lower in all four groups receiving LPS +/- Oct when compared to the control group receiving PN alone. SUN (mg/dL) was significantly higher in all four groups receiving LPS +/- Oct when compared to control. There were no statistically significant differences in nitrogen balance or SUN among the four groups receiving LPS +/- Oct. The ratio of urinary 3-methylhistidine/ creatinine was significantly higher in the LPS + Oct 1000 group compared to the PN group (0.77 +/- 0.37 vs. 0.42 +/- 0.24, p < 0.05). Serum glucose concentrations and incidence of glycosuria among the five groups were not significantly different.
Endotoxin significantly reduces nitrogen balance compared to controls fed PN. Octreotide does not significantly improve nitrogen retention or glucose homeostasis in endotoxemic parenterally fed rats.
本研究比较了不同剂量的奥曲肽对同时接受脂多糖和肠外营养输注的大鼠葡萄糖和蛋白质稳态的影响。
66只雄性Sprague Dawley大鼠（体重185至220克）被随机分为仅接受肠外营养（PN）组、PN加每天6毫克/千克大肠杆菌026:B6脂多糖持续输注（LPS）组、PN加LPS加每天10微克/千克奥曲肽（LPS + Oct 10）组、100微克/千克/天（LPS + Oct 100）组或1000微克/千克/天（LPS + Oct 1000）组，持续48小时。在随机分组前，所有动物均接受等热量和等氮量的PN（170千卡/千克/天葡萄糖和1.1克氮/千克/天），除自由饮水外禁食。比较各组之间的氮平衡、尿3 - 甲基组氨酸/肌酐比值、血清葡萄糖浓度和糖尿发生率。血清尿素氮（SUN）变化纳入48小时累积氮平衡。采用方差分析、邓肯多重范围检验和费舍尔精确检验进行统计分析。
与仅接受PN的对照组相比，接受LPS ± 奥曲肽的所有四组氮平衡（毫克/48小时）均显著降低。与对照组相比，接受LPS ± 奥曲肽的所有四组SUN（毫克/分升）均显著升高。接受LPS ± 奥曲肽的四组之间氮平衡或SUN无统计学显著差异。与PN组相比，LPS + Oct 1000组尿3 - 甲基组氨酸/肌酐比值显著更高（0.77 ± 0.37对0.42 ± 0.24，p < 0.05）。五组之间血清葡萄糖浓度和糖尿发生率无显著差异。
与接受PN喂养的对照组相比，内毒素显著降低氮平衡。奥曲肽不能显著改善内毒素血症肠外喂养大鼠的氮潴留或葡萄糖稳态。
