Burke Z D, Morgan H, Murphy D, Carter D A
School of Molecular and Medical Biosciences, Cardiff University of Wales, UK.
Brain Res Mol Brain Res. 1996 Dec;42(2):287-92. doi: 10.1016/s0169-328x(96)00132-5.
Recent studies have established that the RNA coding for the neuropeptide arginine-vasopressin (AVP) is expressed in the neurohypophyseal compartment of the hypothalamo-neurohypophyseal system. In order to determine the molecular mechanisms that direct this novel expression pattern we have now investigated whether an AVP transgene is similarly regulated. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) approach that permits simultaneous analysis of both endogenous and transgene RNA levels, we have demonstrated that RNA derived from a 3.5-kb bovine vasopressin transgene is expressed in the neurohypophysis of transgenic mice, and is up-regulated by a physiological stimulus (salt-loading) in a similar manner to mouse AVP RNA. Sequences conserved between this region of the murine and bovine AVP genes are therefore sufficient to mediate neurohypophyseal expression. These lines of transgenic mice will serve as a model for the delineation of sequences that target expression beyond the neuronal perikaryon.
最近的研究证实，编码神经肽精氨酸加压素（AVP）的RNA在下丘脑 - 神经垂体系统的神经垂体部分表达。为了确定指导这种新表达模式的分子机制，我们现在研究了AVP转基因是否受到类似的调控。使用逆转录 - 聚合酶链反应（RT-PCR）方法可以同时分析内源性和转基因RNA水平，我们已经证明，来自3.5kb牛加压素转基因的RNA在转基因小鼠的神经垂体中表达，并以与小鼠AVP RNA类似的方式受到生理刺激（盐负荷）的上调。因此，小鼠和牛AVP基因该区域之间保守的序列足以介导神经垂体表达。这些转基因小鼠品系将作为一种模型，用于描绘靶向神经元胞体以外表达的序列。
