Moskophidis D, Moskophidis M, Löhler J
Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912, USA.
J Immunol. 1997 Feb 1;158(3):1254-61.
In phylogenetically diverse species with the help of T lymphocytes or soluble factors, viral infections induce the Ag-specific B lymphocytes to proliferate and terminally differentiate into IgM, IgG, IgA, IgD, or IgE Ab-secreting cells. Based on previous studies searching for IgD, it was inferred that serum IgD in the mouse is nearly undetectable, although in other species, e.g., humans, IgD is a measurable component of serum Ig. More recently, new information has been obtained indicating that IgD is secreted in minute quantities during normal B cell differentiation. We observed that IgD is secreted in significantly increased quantities in mice undergoing an acute infection with lymphocytic choriomeningitis virus or vesicular stomatitis virus compared with uninfected animals. A substantial fraction of the observed IgD was found to be virus specific. Using a solid-phase immunoenzymatic technique, virus-specific IgD Ab-forming cells were detected in the spleen; their numerical increase correlated with the level of secreted antiviral IgD. In addition, immunohistochemical staining revealed IgD+ plasma cells that occurred with a similar kinetic profile as the virus-specific IgD Ab-forming cells. These findings provide direct evidence that synthesis of IgD is a physiologic event in the mouse. Its precise function in the immune response to pathogens, however, remains to be determined.
在系统发育上不同的物种中，在T淋巴细胞或可溶性因子的帮助下，病毒感染诱导抗原特异性B淋巴细胞增殖并最终分化为分泌IgM、IgG、IgA、IgD或IgE抗体的细胞。基于先前对IgD的研究推断，小鼠血清中的IgD几乎无法检测到，尽管在其他物种（如人类）中，IgD是血清Ig的可测量成分。最近，获得了新的信息，表明在正常B细胞分化过程中会分泌微量的IgD。我们观察到，与未感染的动物相比，感染淋巴细胞性脉络丛脑膜炎病毒或水疱性口炎病毒的小鼠分泌的IgD量显著增加。发现观察到的大部分IgD具有病毒特异性。使用固相免疫酶技术，在脾脏中检测到病毒特异性IgD抗体形成细胞；它们数量的增加与分泌的抗病毒IgD水平相关。此外，免疫组织化学染色显示IgD+浆细胞的出现动力学与病毒特异性IgD抗体形成细胞相似。这些发现提供了直接证据，表明IgD的合成在小鼠中是一种生理事件。然而，其在针对病原体的免疫反应中的精确功能仍有待确定。
