Helten A, Marschall M, Reininger A J, Meier-Ewert H
Abeilung für Virologie, Technischen Universität München, Germany.
Acta Virol. 1996 Sep;40(4):223-6.
A persistent variant of influenza C virus was used to infect chickens by intraamniotic (i.a.) inoculation. The infected hatchings were analyzed for virus production in different tissues and for the continuous presence of viral RNA genomes. The permissiveness for infection was demonstrated primarily for the chicken lung, besides other organs. Viral antigens could be detected by indirect immunofluorescence staining for a period of 8 days and reisolates were obtained mainly at early time points post infection (p.i.). Nested reverse transcription-polymerase chain reaction (RT-PCR) directed to 3 genomic sequences was positive at least until day 53, whereby no distinct end point was determined. These experiments provide first evidence for the long-term stability of influenza C virus RNA segments in vivo.
使用一种持续存在的丙型流感病毒变异株通过羊膜内（i.a.）接种感染鸡。对受感染的雏鸡进行分析，以检测不同组织中的病毒产生情况以及病毒RNA基因组的持续存在情况。除其他器官外，主要证明鸡肺对感染具有易感性。通过间接免疫荧光染色可在8天内检测到病毒抗原，并且主要在感染后（p.i.）的早期时间点获得病毒重分离株。针对3个基因组序列的巢式逆转录聚合酶链反应（RT-PCR）至少在第53天呈阳性，因此未确定明显的终点。这些实验为丙型流感病毒RNA片段在体内的长期稳定性提供了首个证据。
