卡比多巴对多巴胺在大鼠纹状体中产生羟自由基的保护作用。

Protective effect of carbidopa on hydroxyl radical generation in the rat striatum by dopamine.

作者信息

Obata T, Yamanaka Y

机构信息

Department of Pharmacology, Oita Medical University, Japan.

出版信息

Neurosci Lett. 1996 Dec 27;221(1):13-6. doi: 10.1016/s0304-3940(96)13271-7.

DOI:10.1016/s0304-3940(96)13271-7

PMID:9014169

Abstract

The effect of L-3,4-dihydroxyphenylalanine (L-DOPA) on the generation of hydroxyl free radicals (.OH) was investigated using striatal microdialysis technique. Salicylic acid in Ringer's solution (0.5 nmol.microliters-1.min-1) was infused directly through a microdialysis probe to detect the generation of .OH as reflected by the formation of dihydroxybenzoic acids (DHBA) in the striatum. When L-DOPA (0.1 mM; 1 microliter.min-1) was infused in the rat brain, the level of 3,4-dihydroxyphenylacetic acid (DOPAC) gradually increased in a time-dependent manner. In addition, a marked elevation of DHBA was observed. However, in the presence of carbidopa, a decarboxylase inhibitor, the elevation in DHBA formation was not observed. These results suggest that carbidopa may suppress the .OH generation by dopamine (DA).

摘要

采用纹状体微透析技术研究了L-3,4-二羟基苯丙氨酸（L-DOPA）对羟自由基（·OH）生成的影响。将林格氏溶液中的水杨酸（0.5 nmol·μL-1·min-1）通过微透析探针直接注入，以检测纹状体中生成的·OH，其通过二羟基苯甲酸（DHBA）的形成来反映。当向大鼠脑内注入L-DOPA（0.1 mM；1 μL·min-1）时，3,4-二羟基苯乙酸（DOPAC）水平呈时间依赖性逐渐升高。此外，还观察到DHBA显著升高。然而，在存在脱羧酶抑制剂卡比多巴的情况下，未观察到DHBA生成的升高。这些结果表明，卡比多巴可能抑制多巴胺（DA）生成·OH。