衰老和阿尔茨海默病大脑中晚期糖基化终产物的免疫组织化学研究

Immunohistochemical study of advanced glycation end products in aging and Alzheimer's disease brain.

作者信息

Takedo A, Yasuda T, Miyata T, Mizuno K, Li M, Yoneyama S, Horie K, Maeda K, Sobue G

机构信息

Department of Neurology, Nagoya University, School of Medicine, Japan.

出版信息

Neurosci Lett. 1996 Dec 27;221(1):17-20. doi: 10.1016/s0304-3940(96)13275-4.

DOI:10.1016/s0304-3940(96)13275-4

PMID:9014170

Abstract

Advanced glycation end products (AGEs) in the brain were immunohistochemically examined in Alzheimer's disease (AD) and aging using anti-AGE antibody recognizing mainly carboxymethyllysine. AGE positive staining diffusely located in the neuronal perikarya of hippocampus and parahippocampus in AD and aged brains without dementia, but not in young brains less than 17 years of age. Extra-neuroperikaryal AGE deposits were also detected in the neuropil of AD and aged brains. The extra-neuroperikaryal AGE deposits markedly increased in AD brains as compared to aged brains. These AGE-positive deposits in the neuropil were not related to the senile plaque identified by anti-beta amyloid protein antibody. These findings suggest a potential link of AGE accumulation in the central nervous system to the aging process of neurons and the degenerating process of AD neurons.

摘要

使用主要识别羧甲基赖氨酸的抗晚期糖基化终产物（AGEs）抗体，通过免疫组织化学方法检测了阿尔茨海默病（AD）患者大脑中的AGEs以及衰老过程中的AGEs情况。在AD患者和无痴呆的老年大脑中，AGE阳性染色弥漫分布于海马体和海马旁回的神经元胞体中，但在17岁以下的年轻大脑中未发现。在AD和老年大脑的神经纤维网中也检测到了神经胞体外AGE沉积物。与老年大脑相比，AD大脑中的神经胞体外AGE沉积物明显增加。这些神经纤维网中的AGE阳性沉积物与抗β淀粉样蛋白抗体识别的老年斑无关。这些发现表明，中枢神经系统中AGE的积累与神经元的衰老过程以及AD神经元的退化过程之间可能存在联系。