日本脑炎病毒免疫刺激复合物（ISCOMs）的制备与表征

Preparation and characterization of immunostimulating complexes (ISCOMs) of Japanese encephalitis virus.

作者信息

Yeolekar L R, Banerjee K

机构信息

National Institute of Virology, Maharashtra, India.

出版信息

Vaccine. 1996 Nov;14(16):1553-9. doi: 10.1016/s0264-410x(96)00091-6.

DOI:10.1016/s0264-410x(96)00091-6

PMID:9014299

Abstract

ISCOMs (immunostimulating complexes) were prepared from envelope glycoprotein (Egp) of Japanese encephalitis (JE) virus. ISCOMs showed a single band of the viral Egp in SDS-PAGE, which reacted with polyclonal and monoclonal antibody (MAb) raised against Egp. Comparison between the epitopes exposed on JE virion and JE ISCOMs, by antigen capture ELISA, utilizing a panel of domain-specific MAbs, revealed identical epitopes exposed on the Egp incorporated in ISCOMs and the whole virion. Electron micrographs of ISCOMs showed spherical cage-like structures of 35 nm. ISCOMs with Egp were good immunogenes, which stimulated high titres of neutralizing antibodies, both in mice and rabbits.

摘要

免疫刺激复合物（ISCOMs）由日本脑炎（JE）病毒的包膜糖蛋白（Egp）制备而成。ISCOMs在SDS-PAGE中显示出病毒Egp的单一条带，它能与针对Egp产生的多克隆抗体和单克隆抗体（MAb）发生反应。利用一组结构域特异性单克隆抗体，通过抗原捕获ELISA对JE病毒粒子和JE ISCOMs上暴露的表位进行比较，结果显示ISCOMs中掺入的Egp和整个病毒粒子上暴露的表位相同。ISCOMs的电子显微照片显示出35纳米的球形笼状结构。含有Egp的ISCOMs是良好的免疫原，在小鼠和兔子体内均能刺激产生高滴度的中和抗体。