Wang L J, Brännström M, Cui K H, Simula A P, Hart R P, Maddocks S, Norman R J
Department of Obstetrics and Gynaecology, Queen Elizabeth Hospital, University of Adelaide, Woodville, Australia.
J Endocrinol. 1997 Jan;152(1):11-7. doi: 10.1677/joe.0.1520011.
Interleukin-1 (IL-1) is a multifunctional cytokine with profound effects on ovarian function. The effects of IL-1 on ovarian steroidogenesis have been demonstrated in several species. IL-1 mRNA levels are increased in the thecal layer of the ovulating follicle and IL-1 beta has been shown to induce ovulations in vitro. In this study we have investigated the presence and distribution of the mRNAs for type I IL-1 receptor (IL-1RtI) and for the naturally occurring IL-1 receptor antagonist (IL-1ra) in ovaries of adult cycling rats, to elucidate the target cells for IL-1 action. We have demonstrated the presence of mRNA for both substance by in situ hybridisation and reverse transcription PCR. mRNA for IL-1RtI was not found in primordial follicles but was abundant in the granulosa and thecal layer in developing follicles with stronger signals in the granulosa layer. In the preovulatory and ovulatory follicles, there was a further increase in the signal for IL-1RtI mRNA in the thecal layer compared with the granulosa layer. Corpora lutea were weakly positive at all stages and atretic follicles were largely negative. No mRNA was detected in oocytes of any stage mRNA for IL-1ra showed a similar distribution to that of IL-1RtI. The changes in distribution suggest an action of IL-1 on rat granulosa cells during follicular development and on thecal cells during ovulation.
白细胞介素-1（IL-1）是一种对卵巢功能有深远影响的多功能细胞因子。IL-1对卵巢甾体激素生成的作用已在多个物种中得到证实。在排卵卵泡的卵泡膜层中，IL-1 mRNA水平升高，并且IL-1β已被证明在体外可诱导排卵。在本研究中，我们调查了成年周期性大鼠卵巢中I型IL-1受体（IL-1RtI）和天然存在的IL-1受体拮抗剂（IL-1ra）的mRNA的存在和分布，以阐明IL-1作用的靶细胞。我们通过原位杂交和逆转录PCR证明了这两种物质的mRNA的存在。在原始卵泡中未发现IL-1RtI的mRNA，但在发育中的卵泡的颗粒层和卵泡膜层中含量丰富，颗粒层中的信号更强。在排卵前和排卵卵泡中，卵泡膜层中IL-1RtI mRNA的信号与颗粒层相比进一步增加。黄体在所有阶段均呈弱阳性，闭锁卵泡大多为阴性。在任何阶段的卵母细胞中均未检测到mRNA。IL-1ra的mRNA显示出与IL-1RtI相似的分布。分布的变化表明IL-1在卵泡发育过程中对大鼠颗粒细胞有作用，在排卵过程中对卵泡膜细胞有作用。
