Piacentini M, Piredda L, Starace D T, Annicchiarico-Petruzzelli M, Mattei M, Oliverio S, Farrace M G, Melino G
Department of Biology, University of Rome Tor Vergata, Italy.
J Pathol. 1996 Dec;180(4):415-22. doi: 10.1002/(SICI)1096-9896(199612)180:4<415::AID-PATH684>3.0.CO;2-A.
This study concerns the role of apoptosis in the growth of human neuroblastomas transplanted into immunodeficient SCID mice. Human neuroblastoma cell lines may consist of one or more distinct phenotypes including the neural 'N-type' and flat substrate-adherent 'S-type'. A differential phenotype-specific proliferation was apparent among S- and N-type cell clones transplanted into SCID mice when compared with the wild-type SK-N-BE(2) cell line. This differential growth capacity of the tumours was correlated with spontaneous apoptosis. Another SK-N-BE(2)-derived cell line (TGA), displaying high levels of apoptosis upon stable transfection with the full length 'tissue' transglutaminase (tTG) cDNA, was unable to induce tumour development when xenografted into SCID mice. To support these observations, the expression of apoptosis-related genes (i.e., bcl-2, p53, and tTG) in the various neuroblastomas was also investigated.
本研究关注凋亡在移植到免疫缺陷SCID小鼠体内的人神经母细胞瘤生长中的作用。人神经母细胞瘤细胞系可能由一种或多种不同表型组成，包括神经“N型”和平板底物贴壁“s型”。与野生型SK-N-BE(2)细胞系相比，移植到SCID小鼠体内的S型和N型细胞克隆之间存在明显的表型特异性增殖差异。肿瘤的这种差异生长能力与自发凋亡相关。另一种源自SK-N-BE(2)的细胞系（TGA），在用全长“组织”转谷氨酰胺酶（tTG）cDNA稳定转染后显示出高水平的凋亡，当异种移植到SCID小鼠体内时无法诱导肿瘤发生。为支持这些观察结果，还研究了各种神经母细胞瘤中凋亡相关基因（即bcl-2、p53和tTG）的表达。
