Scarpato R, Landini E, Migliore L
Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy.
Mutat Res. 1996 Dec;372(2):195-204. doi: 10.1016/s0027-5107(96)00139-x.
The aim of the present paper was to assess the occurrence of acrocentric chromosomes in spontaneous micronuclei (MN) of lymphocytes of 20 subjects (10 males and 10 females) of different ages by means of dual-colour hybridization with pancentromeric and acrocentric-specific DNA probes on the binucleate cells of each subject. MN were found to contain acrocentric chromosome(s) at an average frequency of 26.8%, as compared to a 60% frequency of centromere-positive MN (C + MN). As expected, the percentage of total C + MN increased significantly with increasing age both of all subjects (r = 0.695, p < 0.001) and women (r = 0.814, p < 0.01), while no relationship was found between the frequency of micronuclei containing acrocentric chromosome(s) and donor age. This study indicates that the frequency of MN containing acrocentric chromosomes is neither over-represented nor influenced by age or sex.
本文旨在通过对20名不同年龄受试者（10名男性和10名女性）淋巴细胞自发微核（MN）中的近端着丝粒染色体进行评估，采用双色杂交技术，使用全着丝粒和近端着丝粒特异性DNA探针，对每个受试者的双核细胞进行检测。发现微核中含有近端着丝粒染色体的平均频率为26.8%，而着丝粒阳性微核（C+MN）的频率为60%。正如预期的那样，所有受试者（r=0.695，p<0.001）和女性（r=0.814，p<0.01）的总C+MN百分比均随年龄增长而显著增加，而含有近端着丝粒染色体的微核频率与供体年龄之间未发现相关性。这项研究表明，含有近端着丝粒染色体的微核频率既没有过度表现，也不受年龄或性别的影响。
