大鼠卵巢中两个密切相关基因——诱导型和非诱导型羰基还原酶的鉴定。

Identification of two closely related genes, inducible and noninducible carbonyl reductases in the rat ovary.

作者信息

Aoki H, Okada T, Mizutani T, Numata Y, Minegishi T, Miyamoto K

机构信息

Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University, Japan.

出版信息

Biochem Biophys Res Commun. 1997 Jan 23;230(3):518-23. doi: 10.1006/bbrc.1996.5995.

DOI:10.1006/bbrc.1996.5995

PMID:9015353

Abstract

Two closely related cDNAs encoding carbonyl reductase (CR) were isolated from the rat ovary and testis. One (inducible CR, iCR) was rapidly and strongly induced in the ovary by pregnant mare serum gonadotropin (PMSG), whereas the other (non-inducible CR, nCR) was constitutively expressed in the ovary and was not induced by PMSG. Both genes were also expressed in the rat testis. The cDNAs of rat iCR and nCR encode highly homologous proteins (86% identity in amino acid) with 277 and 276 amino acid residues, respectively. However, the 5'-upstream regions flanking respective genes are completely unrelated to each other except for a short (38 bp) overlap. These results indicate the presence of two closely related but differently regulated CR genes in rat gonadal tissues. Strong induction of iCR by PMSG suggests the importance of the gene in the ovarian follicular development.

摘要

从大鼠卵巢和睾丸中分离出两个编码羰基还原酶（CR）的密切相关的cDNA。其中一个（诱导型CR，iCR）在孕马血清促性腺激素（PMSG）作用下在卵巢中迅速且强烈地被诱导，而另一个（非诱导型CR，nCR）在卵巢中组成性表达且不受PMSG诱导。这两个基因也在大鼠睾丸中表达。大鼠iCR和nCR的cDNA分别编码具有277和276个氨基酸残基的高度同源蛋白（氨基酸同一性为86%）。然而，除了一个短的（38 bp）重叠区域外，各基因侧翼的5'上游区域彼此完全不相关。这些结果表明在大鼠性腺组织中存在两个密切相关但调控方式不同的CR基因。PMSG对iCR的强烈诱导表明该基因在卵巢卵泡发育中具有重要作用。