Toide K, Itoh S, Nagasaka Y, Yanagimoto T, Kamataki T
Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Arch Biochem Biophys. 1997 Feb 1;338(1):43-9. doi: 10.1006/abbi.1996.9792.
A mouse Cyp3a11 gene was isolated from a mouse sperm DNA library with mouse Cyp3a11 cDNA as a probe. The nucleotide sequences determined for the gene and the 5' flanking region revealed that the mouse Cyp3a11 gene was composed of 13 exons and 12 introns. The exons spun about 23 kb. The nucleotide sequence of the exons was completely identical to Cyp3a11 cDNA. Within the 5' flanking sequence, putative binding sites of several transcriptional factors were found. Transient transfection assays were carried out with HepG2 cells, a human hepatoma cell line, using constructs containing different lengths of 5' flanking sequence fused to a reporter, chloramphenicol acetyltransferase gene. The results showed that a cis-acting element(s) was located from -1609 to -907.
以小鼠Cyp3a11 cDNA为探针，从小鼠精子DNA文库中分离出小鼠Cyp3a11基因。对该基因及其5'侧翼区的核苷酸序列分析表明，小鼠Cyp3a11基因由13个外显子和12个内含子组成。外显子约占23 kb。外显子的核苷酸序列与Cyp3a11 cDNA完全相同。在5'侧翼序列中，发现了几个转录因子的假定结合位点。利用含有与氯霉素乙酰转移酶基因（一种报告基因）融合的不同长度5'侧翼序列的构建体，对人肝癌细胞系HepG2细胞进行了瞬时转染分析。结果表明，顺式作用元件位于-1609至-907之间。
