Rochel N, Renaud J P, Ruff M, Vivat V, Granger F, Bonnier D, Lerouge T, Chambon P, Gronemeyer H, Moras D
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, Illkirch, France.
Biochem Biophys Res Commun. 1997 Jan 13;230(2):293-6. doi: 10.1006/bbrc.1996.5787.
A 28-kDa fragment (residues 178-423) of the human retinoic acid receptor gamma, hRARgamma D3E, encompassing the ligand-binding domain (LBD) was overproduced in Escherichia coli and purified as a monomer to more than 95% purity and homogeneity. The Kd for all-trans retinoic acid binding was 0.6 +/- 0.1 nM. Crystals of the LBD complexed with all-trans retinoic acid were grown at pH 7 from sodium acetate in the presence of detergents using the vapor diffusion method. They diffract to 2.0 A using a synchrotron radiation (lambda=0.91 A) and belong to the tetragonal space group P4(1)2(1)2 with unit cell parameters a=b=60.6 A and c=155.3 A, one monomer per asymmetric unit, a solvent content of ca. 33%, and a Vm value of approximately 2 A3/dalton.
人视黄酸受体γ的28 kDa片段（第178 - 423位氨基酸残基），即hRARγ D3E，包含配体结合结构域（LBD），在大肠杆菌中大量表达，并以单体形式纯化，纯度和均一性超过95%。全反式视黄酸结合的解离常数（Kd）为0.6±0.1 nM。采用气相扩散法，在pH 7的条件下，于醋酸钠中并在去污剂存在的情况下，培养出与全反式视黄酸复合的LBD晶体。使用同步辐射（波长λ = 0.91 Å）时，这些晶体的衍射分辨率达到2.0 Å，属于四方晶系空间群P4(1)2(1)2，晶胞参数a = b = 60.6 Å，c = 155.3 Å，每个不对称单位含一个单体，溶剂含量约为33%，Vm值约为2 ų/道尔顿。
