鲟鱼阿黑皮素原含有γ-促黑素的残余部分。

Sturgeon proopiomelanocortin has a remnant of gamma-melanotropin.

作者信息

Amemiya Y, Takahashi A, Dores R M, Kawauchi H

机构信息

Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Sanriku, Iwate, Japan.

出版信息

Biochem Biophys Res Commun. 1997 Jan 13;230(2):452-6. doi: 10.1006/bbrc.1996.5987.

DOI:10.1006/bbrc.1996.5987

PMID:9016801

Abstract

For the investigation of the evolution of the proopiomelanocortin (POMC) gene in early ray-finned fishes, nucleotide sequence of POMC cDNA from a chondrostean fish, the sturgeon has been determined. POMC cDNA was amplified by PCR from double-strand cDNA prepared from sturgeon pituitary and ligated with lambdaZAP II. The POMC cDNA consists of 1079 bp without a poly-A. An open reading frame of the POMC cDNA encodes 263 amino acid residues. Sturgeon POMC contains ACTH, alpha-melanotropin (MSH), beta-MSH and beta-END at positions (115-153), (115-127), (186-202) and (205-238), respectively. Location of POMC(51-72) is homologous to gamma-MSH, whereas the third residue of MSH-core sequence, His-Phe-Arg-Trp, is changed to His. Moreover, there are no basic amino acids to serve as a processing signal on the N-terminal side of POMC(51-72). These structural characteristics suggest that an ancestor of the ray-finned fishes had gamma-MSH, whereas significant mutations occurred during the evolution of chondrostean fish.

摘要

为了研究早期硬骨鱼类中阿黑皮素原（POMC）基因的进化，已测定了一种软骨硬鳞鱼——鲟鱼的POMC cDNA核苷酸序列。通过PCR从鲟鱼脑垂体制备的双链cDNA中扩增出POMC cDNA，并将其与λZAP II连接。该POMC cDNA由1079个碱基对组成，无poly - A。POMC cDNA的一个开放阅读框编码263个氨基酸残基。鲟鱼POMC在位置（115 - 153）、（115 - 127）、（186 - 202）和（205 - 238）分别包含促肾上腺皮质激素（ACTH）、α - 促黑素（MSH）、β - MSH和β - 内啡肽（β - END）。POMC（51 - 72）的位置与γ - MSH同源，而MSH核心序列的第三个残基His - Phe - Arg - Trp变为His。此外，在POMC（51 - 72）的N端侧没有作为加工信号的碱性氨基酸。这些结构特征表明硬骨鱼类的一个祖先具有γ - MSH，而在软骨硬鳞鱼的进化过程中发生了显著突变。