Suppr超能文献

肌球蛋白SH1和SH2烷基化的图形评估：N-苯基马来酰亚胺反应

Graphical evaluation of alkylation of myosin's SH1 and SH2: the N-phenylmaleimide reaction.

作者信息

Xie L, Li W X, Barnett V A, Schoenberg M

机构信息

Laboratory of Physical Biology, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Biophys J. 1997 Feb;72(2 Pt 1):858-65. doi: 10.1016/s0006-3495(97)78720-4.

DOI:10.1016/s0006-3495(97)78720-4
PMID:9017211
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1185609/
Abstract

Previous assertions about the effect of alkylation of SH1 and SH2 on the myosin high-salt calcium and EDTA ATPases have been summarized, and a simple procedure for obtaining the fractional labeling of SH1 and SH2 after treatment of myosin with alkylating agents has been derived. A simple graphical procedure for illustrating the degree of preference of a particular alkylating agent for SH1 over SH2 has also been developed. The procedures we developed were validated by applying them to two previously studied compounds, 4-(2-iodoacetamido)-TEMPO and 2,4-dinitrofluorobenzine, and then were used to determine a procedure for maximizing the extent of labeling of SH1 alone by N-phenylmaleimide, a compound not previously studied in this manner. It was found that approximately 80% of the SH1 sites could be alkylated without significant alkylation of SH2.

摘要

关于SH1和SH2烷基化对肌球蛋白高盐钙和EDTA ATP酶的影响，之前已有相关论断进行了总结，并且还得出了一种简单的方法，用于在肌球蛋白用烷基化剂处理后获得SH1和SH2的部分标记。还开发了一种简单的图形方法，用于说明特定烷基化剂对SH1相对于SH2的偏好程度。我们开发的方法通过应用于两种先前研究过的化合物4-(2-碘乙酰胺基)-TEMPO和2,4-二硝基氟苯进行了验证，然后用于确定一种方法，以最大限度地提高N-苯基马来酰亚胺单独对SH1的标记程度，N-苯基马来酰亚胺是一种此前未以这种方式研究过的化合物。结果发现，大约80%的SH1位点可以被烷基化，而SH2没有明显的烷基化。

相似文献

1
Graphical evaluation of alkylation of myosin's SH1 and SH2: the N-phenylmaleimide reaction.肌球蛋白SH1和SH2烷基化的图形评估：N-苯基马来酰亚胺反应
Biophys J. 1997 Feb;72(2 Pt 1):858-65. doi: 10.1016/s0006-3495(97)78720-4.
2
Binding of SH1-SH2-modified myosin subfragment-1 to actin.SH1-SH2修饰的肌球蛋白亚片段-1与肌动蛋白的结合。
Biochemistry. 1998 Jun 2;37(22):8048-53. doi: 10.1021/bi980319k.
3
Catalytic cooperativity induced by SH1 labeling of myosin filaments.肌球蛋白丝的SH1标记诱导的催化协同作用。
Biochemistry. 1991 Jan 8;30(1):286-94. doi: 10.1021/bi00215a039.
4
Binding of F-actin to a region between SH1 and SH2 groups of myosin subfragment-1 which may determine the high affinity of acto-subfragment-1 complex at rigor.F-肌动蛋白与肌球蛋白亚片段-1的SH1和SH2结构域之间的区域结合，这可能决定了在僵直状态下肌动蛋白-亚片段-1复合物的高亲和力。
J Biochem. 1984 Feb;95(2):447-54. doi: 10.1093/oxfordjournals.jbchem.a134626.
5
Thiol-specific cross-linkers of variable length reveal a similar separation of SH1 and SH2 in myosin subfragment 1 in the presence and absence of MgADP.不同长度的硫醇特异性交联剂表明，在有和没有MgADP的情况下，肌球蛋白亚片段1中的SH1和SH2有类似的分离。
Biochemistry. 1999 Aug 10;38(32):10307-17. doi: 10.1021/bi990615c.
6
The reactive SH1 and SH2 cysteines in myosin subfragment 1 are cross-linked at similar rates with reagents of different length.
Biochem Biophys Res Commun. 1982 May 14;106(1):117-22. doi: 10.1016/0006-291x(82)92066-6.
7
Probing the conformational states of the SH1-SH2 helix in myosin: a cross-linking approach.探究肌球蛋白中SH1-SH2螺旋的构象状态：一种交联方法。
Biochemistry. 1998 Nov 24;37(47):16704-10. doi: 10.1021/bi9817212.
8
Location of SH1 and SH2 along a heavy chain of myosin subfragment 1.肌球蛋白亚片段1重链上SH1和SH2的位置。
Biochemistry. 1981 May 26;20(11):3281-5. doi: 10.1021/bi00514a046.
9
An investigation into the role of SH1 and SH2 groups of myosin in calcium binding and tension generation.对肌球蛋白的SH1和SH2基团在钙结合及张力产生中的作用的研究。
Biochem Biophys Res Commun. 1980 Feb 27;92(4):1383-8. doi: 10.1016/0006-291x(80)90439-8.
10
Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.通过一种新的硫醇间交联作用使核苷酸捕获在肌球蛋白亚片段1的ATP酶位点上。
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2037-41. doi: 10.1073/pnas.83.7.2037.

引用本文的文献

1
Structural characterization of weakly attached cross-bridges in the A*M*ATP state in permeabilized rabbit psoas muscle.通透化兔腰大肌中处于A*M*ATP状态的弱附着横桥的结构表征
Biophys J. 2002 Apr;82(4):2111-22. doi: 10.1016/S0006-3495(02)75558-6.
2
Is SH1-SH2-cross-linked myosin subfragment 1 a structural analog of the weakly-bound state of myosin?SH1-SH2交联的肌球蛋白亚片段1是肌球蛋白弱结合状态的结构类似物吗？
Biophys J. 2000 Jul;79(1):460-7. doi: 10.1016/S0006-3495(00)76307-7.
3
Behavior of N-phenylmaleimide-reacted muscle fibers in magnesium-free rigor solution.N-苯基马来酰亚胺反应的肌纤维在无镁僵直溶液中的行为。
Biophys J. 1998 Mar;74(3):1110-4. doi: 10.1016/S0006-3495(98)77829-4.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Saturation transfer electron parametric resonance of an indane-dione spin-label. Calibration with hemoglobin and application to myosin rotational dynamics.茚满二酮自旋标记物的饱和转移电子顺磁共振。用血红蛋白进行校准并应用于肌球蛋白旋转动力学。
Biophys J. 1993 Jun;64(6):1896-907. doi: 10.1016/S0006-3495(93)81561-3.
3
A spin label that binds to myosin heads in muscle fibers with its principal axis parallel to the fiber axis.一种自旋标记物，其主轴与肌纤维轴平行，可与肌纤维中的肌球蛋白头部结合。
Biophys J. 1994 Oct;67(4):1634-45. doi: 10.1016/S0006-3495(94)80636-8.
4
The site and stoichiometry of the N-phenylmaleimide reaction with myosin when weakly-binding crossbridges are formed in skinned rabbit psoas fibers.当在去表皮的兔腰大肌纤维中形成弱结合横桥时，N-苯基马来酰亚胺与肌球蛋白反应的位点和化学计量关系。
Biochim Biophys Acta. 1995 Nov 21;1232(1-2):13-20. doi: 10.1016/0005-2728(95)00094-6.
5
Submillisecond rotational dynamics of spin-labeled myosin heads in myofibrils.肌原纤维中自旋标记肌球蛋白头部的亚毫秒级旋转动力学。
Biophys J. 1980 Dec;32(3):873-89. doi: 10.1016/S0006-3495(80)85023-5.
6
Sulfhydryl modification and labeling of myosin.肌球蛋白的巯基修饰与标记
Methods Enzymol. 1982;85 Pt B:84-93. doi: 10.1016/0076-6879(82)85012-x.
7
The effect of myosin sulphydryl modification on the mechanics of fibre contraction.肌球蛋白巯基修饰对纤维收缩力学的影响。
J Muscle Res Cell Motil. 1984 Apr;5(2):131-46. doi: 10.1007/BF00712152.
8
Effect of nucleotides and pyrophosphate on spin labels bound to S1 thiol groups of myosin.核苷酸和焦磷酸对与肌球蛋白S1巯基结合的自旋标记物的影响。
Biochemistry. 1970 Aug 4;9(16):3265-72. doi: 10.1021/bi00818a021.
9
Cooperative role of two sulfhydryl groups in myosin adenosine triphosphatase.两个巯基在肌球蛋白三磷酸腺苷酶中的协同作用。
Biochemistry. 1974 May 7;13(10):2014-22. doi: 10.1021/bi00707a003.
10
Sulfhydryl groups involved in the active site of myosin A adenosine triphosphatase. I. Specific blocking of the SH group responsible for the inhibitory phase in "B phasic response" of the catalytic activity.参与肌球蛋白A三磷酸腺苷酶活性位点的巯基。I. 对催化活性“B相反应”中负责抑制阶段的巯基进行特异性阻断。
J Biochem. 1966 Jan;59(1):24-33. doi: 10.1093/oxfordjournals.jbchem.a128254.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验