肌球蛋白SH1和SH2烷基化的图形评估：N-苯基马来酰亚胺反应
Graphical evaluation of alkylation of myosin's SH1 and SH2: the N-phenylmaleimide reaction.
作者信息
Xie L, Li W X, Barnett V A, Schoenberg M
机构信息
Laboratory of Physical Biology, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
出版信息
Biophys J. 1997 Feb;72(2 Pt 1):858-65. doi: 10.1016/s0006-3495(97)78720-4.
Previous assertions about the effect of alkylation of SH1 and SH2 on the myosin high-salt calcium and EDTA ATPases have been summarized, and a simple procedure for obtaining the fractional labeling of SH1 and SH2 after treatment of myosin with alkylating agents has been derived. A simple graphical procedure for illustrating the degree of preference of a particular alkylating agent for SH1 over SH2 has also been developed. The procedures we developed were validated by applying them to two previously studied compounds, 4-(2-iodoacetamido)-TEMPO and 2,4-dinitrofluorobenzine, and then were used to determine a procedure for maximizing the extent of labeling of SH1 alone by N-phenylmaleimide, a compound not previously studied in this manner. It was found that approximately 80% of the SH1 sites could be alkylated without significant alkylation of SH2.
关于SH1和SH2烷基化对肌球蛋白高盐钙和EDTA ATP酶的影响，之前已有相关论断进行了总结，并且还得出了一种简单的方法，用于在肌球蛋白用烷基化剂处理后获得SH1和SH2的部分标记。还开发了一种简单的图形方法，用于说明特定烷基化剂对SH1相对于SH2的偏好程度。我们开发的方法通过应用于两种先前研究过的化合物4-(2-碘乙酰胺基)-TEMPO和2,4-二硝基氟苯进行了验证，然后用于确定一种方法，以最大限度地提高N-苯基马来酰亚胺单独对SH1的标记程度，N-苯基马来酰亚胺是一种此前未以这种方式研究过的化合物。结果发现，大约80%的SH1位点可以被烷基化，而SH2没有明显的烷基化。
Zotero 插件