Tohya Y, Yokoyama N, Maeda K, Kawaguchi Y, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, Kagoshima University, Japan.
J Gen Virol. 1997 Feb;78 ( Pt 2):303-5. doi: 10.1099/0022-1317-78-2-303.
In order to locate amino acid residues involved in the formation of feline calicivirus (FCV) neutralizing epitopes, we analysed the capsid protein gene of monoclonal antibody neutralization-resistant variants of FCV. Amino acid substitutions in the variants were identified in the two hypervariable regions of the capsid protein. Four linear and two conformational epitopes were located in the regions from residues 426 to 460 and 490 to 520, respectively. The relative positions of individual epitopes agreed with our previous antigenic analysis. Two antigenic sites composed of the neutralizing epitopes were mapped in the hypervariable regions of the capsid protein, demonstrating that a relationship exists between the genetic variability and antigenic differences in the neutralization of FCV.
为了定位参与猫杯状病毒（FCV）中和表位形成的氨基酸残基，我们分析了FCV单克隆抗体中和抗性变体的衣壳蛋白基因。在衣壳蛋白的两个高变区鉴定出了变体中的氨基酸取代。四个线性表位和两个构象表位分别位于第426至460位残基和第490至520位残基的区域。各个表位的相对位置与我们之前的抗原分析结果一致。由中和表位组成的两个抗原位点定位在衣壳蛋白的高变区，这表明在FCV中和过程中，遗传变异性与抗原差异之间存在关联。
