Hernández C, Visser P B, Brown D J, Bol J F
Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden, The Netherlands.
J Gen Virol. 1997 Feb;78 ( Pt 2):465-7. doi: 10.1099/0022-1317-78-2-465.
Tobacco rattle virus isolate PpK20 is transmitted by the nematode Paratrichodorus pachydermus. RNA 2 of the virus determines vector transmissibility and encodes the viral coat protein and two non-structural proteins with molecular masses of 29.4 kDa and 32.8 kDa. Deletions and a frameshift in the two non-structural genes did not interfere with encapsidation or co-replication of RNA 2 with RNA 1 after mechanical inoculation of plants. Mutations that affected the 29.4K gene or both non-structural genes abolished nematode transmission, whereas a large deletion in the 32.8K gene had no effect on transmission by P. pachydermus. It is concluded that the 29.4K gene but not the 32.8K gene is involved in transmission of isolate PpK20 by this vector.
烟草脆裂病毒分离株PpK20由线虫厚皮拟毛刺线虫传播。该病毒的RNA 2决定了介体传播能力，并编码病毒外壳蛋白以及分子量分别为29.4 kDa和32.8 kDa的两种非结构蛋白。在对植物进行机械接种后，两个非结构基因中的缺失和移码并不影响RNA 2与RNA 1的衣壳化或共复制。影响29.4K基因或两个非结构基因的突变消除了线虫传播能力，而32.8K基因中的大片段缺失对厚皮拟毛刺线虫的传播没有影响。得出的结论是，29.4K基因而非32.8K基因参与了该介体对分离株PpK20的传播。
